Genetic and transcriptional analysis of flgB flagellar operon constituents in the oral spirochete Treponema denticola and their heterologous expression in enteric bacteria.

Infection and Immunity
H F HeinzerlingR A Burne

Abstract

Oral spirochetes possess many potential virulence factors, including the capacity for tissue invasion and persistence despite a vigorous host immune response. In an attempt to identify treponemal immunoreactive components, sera derived from individuals with advanced periodontal disease were used as a reagent to isolate recombinant bacteriophage lambda clones expressing antigens of the oral spirochete Treponema denticola ATCC 35405. Nucleotide sequence analysis of a clone expressing three immunoreactive products has revealed seven T. denticola genes which appear to encode homologs of flagellar basal body constituents, FlgB, FlgC, FliE, and FliF, a flagellar switch component, FliG, and the putative flagellar export proteins, FliH and FliI, initially characterized in Salmonella typhimurium. Also identified was a gene resembling fliJ. Primer extension analysis identified a transcriptional start site 5' to the treponemal flgB gene. Appropriately spaced with respect to this start site was a sigma28 binding motif. The absence of additional identifiable sigma factor binding motifs within the treponemal sequence and the proximity of adjacent genes suggested operonic arrangement, and reverse transcriptase PCR provided evidence of cotrans...Continue Reading

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Citations

Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·M A MotalebN W Charon
Sep 10, 1999·Microbiology and Immunology·K Ishihara, K Okuda
Apr 28, 2000·Microbiology·A TasteyreP Borriello
Mar 27, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·L V Stamm, H L Bergen
Aug 5, 2006·Anaerobe·J C Fenno, B C McBride

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