Genetic assessment of the internal transcribed spacer region (ITS1.2) in Mangifera indica L. landraces

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Bahman Fazeli-NasabHesham Ali El-Enshasy

Abstract

Mango (Mangifera indica) is one of the most important tropical fruits in the world. Twenty-two genotypes of native mangoes from different regions of southern Iran (Hormozgan and Kerman) were collected and analyzed for the ribosomal genes. GC content was found to be 55.5%. Fu and Li's D* test statistic (0.437), Fu and Li's F* test statistic (0.500) and Tajima's D (1.801) were positive and nonsignificant. A total of 769 positions were identified (319 with insertion or deletion including 250 polymorphic and 69 monomorphic loci; 450 loci without any insertion or deletion including 35 Singletons and 22 haplotypes). Nucleotide diversity of 0.309 and a high genetic differentiation including Chi square of 79.8; P value of 0.3605 and df value of 76 was observed among mango genotypes studied. The numerical value of the ratio dN/dS (0.45) indicated a pure selection in the examined gene and the absence of any key changes. Cluster analysis differentiated the mango used in this research (M. indica L.) into two genotypes but could not differentiate their geographical locations. The results of this study indicated that a high genetic distance exists between HajiGholam (Manojan) and Arbabi (Rodan) genotypes and showed higher genetic diversity i...Continue Reading

References

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May 8, 2018·Applications in Plant Sciences·Michael R McKainYa Yang

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Methods Mentioned

BETA
PCR

Software Mentioned

Nebcutter
MEGA6
DnaSP5
ClustalW
Clustal
BioEdit Sequence Alignment Editor
Dnasp
DNASTAR
GenScript
BLAST

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