Genetic basis for Rhizobium etli CE3 O-antigen O-methylated residues that vary according to growth conditions.

Journal of Bacteriology
Kristylea J OjedaK Dale Noel

Abstract

The Rhizobium etli CE3 O antigen is a fixed-length heteropolymer with O methylation being the predominant type of sugar modification. There are two O-methylated residues that occur, on average, once per complete O antigen: a multiply O-methylated terminal fucose and 2-O methylation of a fucose residue within a repeating unit. The amount of the methylated terminal fucose decreases and the amount of 2-O-methylfucose increases when bacteria are grown in the presence of the host plant, Phaseolus vulgaris, or its seed exudates. Insertion mutagenesis was used to identify open reading frames required for the presence of these O-methylated residues. The presence of the methylated terminal fucose required genes wreA, wreB, wreC, wreD, and wreF, whereas 2-O methylation of internal fucoses required the methyltransferase domain of bifunctional gene wreM. Mutants lacking only the methylated terminal fucose, lacking only 2-O methylation, or lacking both the methylated terminal fucose and 2-O methylation exhibited no other lipopolysaccharide structural defects. Thus, neither of these decorations is required for normal O-antigen length, transport, or assembly into the final lipopolysaccharide. This is in contrast to certain enteric bacteria in...Continue Reading

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Citations

Feb 26, 2013·Journal of Bacteriology·Kristylea J OjedaK Dale Noel
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Mar 8, 2011·Molecular Plant-microbe Interactions : MPMI·Jodie Box, K Dale Noel
May 13, 2014·The Journal of Biological Chemistry·Tiezheng LiK Dale Noel

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