Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase

Journal of Bacteriology
Thomas D MandWilliam W Metcalf

Abstract

The methanogenic archaeon Methanosarcina barkeri encodes three distinct types of hydrogenase, whose functions vary depending on the growth substrate. These include the F420-dependent (Frh), methanophenazine-dependent (Vht), and ferredoxin-dependent (Ech) hydrogenases. To investigate their physiological roles, we characterized a series of mutants lacking each hydrogenase in various combinations. Mutants lacking Frh, Vht, or Ech in any combination failed to grow on H2-CO2, whereas only Vht and Ech were essential for growth on acetate. In contrast, a mutant lacking all three grew on methanol with a final growth yield similar to that of the wild type and produced methane and CO2 in the expected 3:1 ratio but had a ca. 33% lower growth rate. Thus, hydrogenases play a significant, but nonessential, role during growth on this substrate. As previously observed, mutants lacking Ech failed to grow on methanol-H2 unless they were supplemented with biosynthetic precursors. Interestingly, this phenotype was abolished in the Δech Δfrh and Δech Δfrh Δvht mutants, consistent with the idea that hydrogenases inhibit methanol oxidation in the presence of H2, which prevents production of the reducing equivalents needed for biosynthesis. Quantifica...Continue Reading

References

Sep 1, 1989·European Journal of Biochemistry·K Fiebig, B Friedrich
Sep 5, 1986·Journal of Molecular Biology·B L Wanner
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W MetcalfR S Wolfe
Mar 28, 1998·Archives of Microbiology·M Vaupel, R K Thauer
Sep 22, 1999·European Journal of Biochemistry·J MeuerR Hedderich
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jörn MeuerWilliam W Metcalf
Jun 1, 2004·Journal of Bioenergetics and Biomembranes·Uwe Deppenmeier
Nov 1, 1993·Applied and Environmental Microbiology·K R SowersR P Gunsalus
Aug 31, 2007·Biostatistics·Mark D Robinson, Gordon K Smyth
Apr 2, 2008·Annals of the New York Academy of Sciences·Yuchen Liu, William B Whitman
Jul 1, 2008·Nature Reviews. Microbiology·Rudolf K ThauerReiner Hedderich
Dec 5, 2008·Archaea : an International Microbiological Journal·Adam M GussWilliam W Metcalf
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gargi KulkarniWilliam W Metcalf
Mar 20, 2010·Annual Review of Biochemistry·Rudolf K ThauerSeigo Shima
Aug 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Thomas J LieJohn A Leigh
Dec 18, 2013·Biochimica Et Biophysica Acta·Cornelia Welte, Uwe Deppenmeier
Dec 3, 2014·Biochimica Et Biophysica Acta·John W PetersMichael W W Adams
Feb 19, 2015·Journal of Bacteriology·He Fu, William W Metcalf

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Citations

Sep 20, 2019·Microbiology and Molecular Biology Reviews : MMBR·Thomas D Mand, William W Metcalf
Jan 1, 2020·Biotechnology for Biofuels·Pinjing HeFan Lü
Dec 14, 2018·Emerging Topics in Life Sciences·Nicole R Buan
Apr 9, 2021·Frontiers in Microbiology·Kailin Gao, Yahai Lu
May 30, 2021·Environmental Research·Jian LiuLeilei Xiao
May 2, 2021·Applied and Environmental Microbiology·Jinjie ZhouDerek R Lovley

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