Genetic changes that increase 5-hydroxymethyl furfural resistance in ethanol-producing Escherichia coli LY180.

Biotechnology Letters
E N MillerL O Ingram

Abstract

The ability of a biocatalyst to tolerate furan inhibitors present in hemicellulose hydrolysates is important for the production of renewable chemicals. This study shows EMFR9, a furfural-tolerant mutant of ethanologenic E. coli LY180, has also acquired tolerance to 5-hydroxymethyl furfural (5-HMF). The mechanism of action of 5-HMF and furfural appear similar. Furan tolerance results primarily from lower expression of yqhD and dkgA, two furan reductases with a low K(m) for NADPH. Furan tolerance was also increased by adding plasmids encoding a NADPH/NADH transhydrogenase (pntAB). Together, these results support the hypothesis that the NADPH-dependent reduction of furans by YqhD and DkgA inhibits growth by competing with biosynthesis for this limiting cofactor.

References

Aug 10, 1999·Biotechnology and Bioengineering·J ZaldivarL O Ingram
Aug 5, 2000·Applied Microbiology and Biotechnology·M J TaherzadehG Lidén
Aug 10, 2000·Biotechnology Progress·A MartinezL O Ingram
May 2, 2003·Applied Biochemistry and Biotechnology·Byung-Hwan UmLinda Henk
Jul 8, 2005·Applied and Environmental Microbiology·J E PurvisL O Ingram
Dec 13, 2006·Biotechnology Letters·Alfredo MartinezL O Ingram
Mar 12, 2008·Applied Microbiology and Biotechnology·João R M AlmeidaMarie-F Gorwa-Grauslund
Nov 1, 2008·Nucleic Acids Research·Ingrid M KeselerPeter D Karp
Feb 3, 2009·Applied Microbiology and Biotechnology·João R M AlmeidaGunnar Lidén
Aug 18, 2009·Applied and Environmental Microbiology·Elliot N MillerLonnie O Ingram

❮ Previous
Next ❯

Citations

Jul 27, 2011·Applied Microbiology and Biotechnology·Laura R JarboeRobert C Brown
May 18, 2012·Applied Microbiology and Biotechnology·Ming-xiong HeXiao-hong Su
Oct 25, 2012·Applied Microbiology and Biotechnology·André PickVolker Sieber
Jul 2, 2011·Biotechnology Letters·Manoj Agrawal, Rachel Ruizhen Chen
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xuan WangLonnie O Ingram
Jan 24, 2012·Applied and Environmental Microbiology·Xuan WangLonnie O Ingram
Feb 4, 2014·PloS One·Tirzah Y GlebesRyan T Gill
Jul 13, 2013·International Journal of Biological Sciences·Omodele Ibraheem, Bongani K Ndimba
Jan 25, 2016·Journal of Industrial Microbiology & Biotechnology·Jieni LianLaura R Jarboe
Dec 15, 2015·Journal of Industrial Microbiology & Biotechnology·Hyung-Min SeoYung-Hun Yang
May 24, 2011·Current Opinion in Biotechnology·Claudia C GeddesLonnie O Ingram
Mar 22, 2016·Bioresource Technology·Meera ChristopherAshok Pandey
Jun 19, 2013·Molecular Systems Biology·Jeffrey M SkerkerAdam P Arkin
Mar 6, 2015·Frontiers in Bioengineering and Biotechnology·Lizbeth M NievesXuan Wang
May 24, 2016·Trends in Biochemical Sciences·Vijai K GuptaThaddeus Ezeji
May 4, 2017·Biotechnology and Bioengineering·HaiFeng SuFuRong Tan
Jun 14, 2015·Applied and Environmental Microbiology·Tippapha PisithkulDaniel Amador-Noguez
Jan 13, 2019·Applied and Environmental Microbiology·Gavin KurganXuan Wang
Mar 17, 2017·Scientific Reports·Huansheng CaoYing Xu
Apr 12, 2017·Applied Microbiology and Biotechnology·Hideo KawaguchiAhikiko Kondo
Feb 17, 2021·Biotechnology Letters·José Eduardo ArteagaJuan-Carlos Sigala
Feb 23, 2018·Metabolic Engineering Communications·Michael T GuarnieriGregg T Beckham
Mar 10, 2021·Journal of Industrial Microbiology & Biotechnology·K T Shanmugam, Lonnie O Ingram
Sep 30, 2021·Applied and Environmental Microbiology·S Bilal JilaniSyed Shams Yazdani

❮ Previous
Next ❯

Software Mentioned

Graphpad Prism

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.