PMID: 8606178Apr 1, 1996Paper

Genetic characteristics of new recA mutants of Escherichia coli K-12

Journal of Bacteriology
A A AlexseyevV A Lanzov

Abstract

To search for functionally thermosensitive (FT) recA mutations, as well as mutations with differently affect RecA protein functions, seven new recA mutations in three different regions of the RecA protein structure proposed by Story et al. [R. M. Story, I. T. Weber, and T. A. Steitz, Nature (London) 355:318-325, 1992] were constructed. Additionally, the recA2283 allele responsible for the FT phenotype of the recA200 mutant was sequenced. Five single mutations (recA2277, recA2278, recA2283, recA2283E, and recA2284) and one double mutation (recA2278-5) generated, respectively, the amino acid substitutions L-277-->N, G-278-->P, L-283-->P, L-283-->E, I-284-->D, and G-278-->T plus V-275-->F in the alpha-helix H-beta-strand 9 region of the C-terminal domain of the RecA protein structure. According to recombination, repair, and SOS-inducible characteristics, these six mutations fall into four phenotypic classes: (i) an FT class, with either inhibition of all three analyzed functions at 42 degrees C (recA2283), preferable inhibition at 42 degrees C of recombination and the SOS response (recA2278), or inhibition at 42 degrees C of only recombination (recA2278-5); (ii) a moderately deficient class (recA2277); (iii) a nondeficient class (...Continue Reading

References

Jan 23, 1992·Nature·R M StoryT A Steitz
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Jan 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K C Smith, T C Wang
Jan 1, 1986·Advances in Biophysics·H Ogawa, T Ogawa
Jan 1, 1984·Molecular & General Genetics : MGG·H KawashimaH Ogawa
Jan 1, 1981·Molecular & General Genetics : MGG·I D HicksonP T Emmerson
Jan 1, 1981·Molecular & General Genetics : MGG·S E BreslerV A Lanzov
May 1, 1982·Cell·J W Little, D W Mount
Sep 1, 1994·Microbiological Reviews·S C KowalczykowskiW M Rehrauer

❮ Previous
Next ❯

Citations

Mar 3, 2020·Frontiers in Microbiology·Duo-Hong ShengYue-Zhong Li
Dec 25, 2003·Critical Reviews in Biochemistry and Molecular Biology·Dharia A McGrew, Kendall L Knight
Aug 31, 2001·Biochemical and Biophysical Research Communications·M D BergerS F Singleton
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.