Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal.

Virus Research
Ricardo ParreiraAida Esteves

Abstract

We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs. The CTFV genome encodes a single polyprotein with 3357 residues showing all the features expected for those of flaviviruses. Phylogenetic analyses based on all ISF sequences available to date, place CTFV among Culex-associated flaviviruses, grouping with recently published NS5 partial sequences documented from mosquitoes collected in the Iberian Peninsula, and with Quang Binh virus (isolated in Vietnam) as a close relative. No CTFV sequences were found integrated in their host's genome using a range of specific PCR primers designed to the prM/E, NS3, and NS5 region.

References

Nov 1, 1992·The Journal of General Virology·R Bulich, J G Aaskov
Feb 25, 1984·Journal of Molecular Biology·G von Heijne
May 24, 2000·Cellular Signalling·D ChristopheB Pichon
Jun 20, 2001·Journal of Virology·A A KhromykhE G Westaway
Feb 14, 2003·Journal of Virology·Rosa Martha E Yocupicio-MonroyRosa M del Angel
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 24, 2004·The Journal of General Virology·Caroline ThurnerPeter F Stadler
Jun 26, 2004·The Journal of General Virology·Sandrine CrochuXavier de Lamballerie
Mar 11, 2006·The Journal of General Virology·Shelley CookXavier de Lamballerie
May 16, 2006·Journal of Virology·Pradeep Devappa UchilVijaya Satchidanandam
Mar 29, 2008·Briefings in Bioinformatics·Kazutaka Katoh, Hiroyuki Toh
Apr 10, 2008·Molecular Biology and Evolution·David Posada
May 6, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·A P G AlmeidaA Esteves
Apr 7, 2009·Archives of Virology·Mary B CrabtreeBarry R Miller
Jan 1, 2007·Journal of Insect Science·Joel J L LutomiahRosemary C Sang
Sep 22, 2010·Vector Borne and Zoonotic Diseases·António Paulo G AlmeidaAida Esteves
Dec 15, 2010·The Journal of General Virology·Yingfeng LeiAndrew Dayton
Jan 25, 2011·Vector Borne and Zoonotic Diseases·Christina M NewmanTony L Goldberg
Oct 26, 2011·Vector Borne and Zoonotic Diseases·Ana VázquezAntonio Tenorio

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Citations

Jul 19, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anna PapaSpiros Mourelatos
Sep 18, 2015·Viruses·Bethany G BollingNikos Vasilakis
Dec 25, 2012·Virus Research·Covadonga AlonsoRaquel Muñoz-Moreno
Oct 19, 2014·The Journal of General Virology·Michela GrisentiAnnapaola Rizzoli
Aug 4, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mattia CalzolariMichele Dottori
Dec 18, 2013·Virus Research·Shuqing ZuoJiusong Zhang
Sep 25, 2012·Virology·Eili HuhtamoXavier de Lamballerie
Sep 26, 2014·Archives of Virology·Alex Pauvolid-CorrêaNicholas Komar
Nov 20, 2015·Journal of Immunology Research·Juan Santiago Salas-Benito, Mónica De Nova-Ocampo
Sep 30, 2016·PloS One·Harindranath CholletiAnne-Lie Blomström
Dec 13, 2019·Science Translational Medicine·Jody Hobson-PetersRoy A Hall
Mar 23, 2017·Parasites & Vectors·Koray ErgünayYvonne-Marie Linton
Nov 15, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Koray ErgünayYvonne-Marie Linton

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Datasets Mentioned

BETA
AY457040
AB377213
EU879060
FJ502995
HOU24518
FJ644291
FJ663034
GQ165808
HE574573
HE574574

Methods Mentioned

BETA
electron microscopy
PCR
dissection

Software Mentioned

BioEdit
NetPhosK
Motif
NetNGlyco
jModeltest
CAP Contig Manager
BLASTn
FigTree
TMHMM Server
- ID ( Barcode of Life Data System Identification engine

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