Genetic characterization of Echinococcus granulosus strains isolated from humans based on nad1 and cox1 gene analysis in Isfahan, central Iran

Journal of Helminthology
R JafariH Yousofi Darani

Abstract

Cystic echinococcosis (CE) is a medically important parasite-caused human disease. Humans may acquire the infection accidentally by ingestion of E. granulosus eggs. The parasite has a broad range of hosts and genotypes, which may affect its aetiological and biological characteristics. The present study aimed to determine the genetic characteristics of human isolates of E. granulosus in Isfahan, Iran. In this cross-sectional study, 50 surgically removed hydatid cysts were collected from hospitalized patients in Al-Zahra Hospital, Isfahan, Iran, over a period of 2 years (2015-2017). DNA was extracted from cyst material, and polymerase chain reactions (PCR) were performed targeting cox1 and nad1 genes. Amplicons were sequenced directly and the resulting sequences were aligned and analysed. Phylogenetic and genetic diversity analyses were also performed. Among the isolates, 43 (86%), 3 (6%) and 4 (8%) out of 50 were E. granulosus (G1), E. granulosus (G3) and E. intermedius (G6), respectively. In total, nine and eight haplotypes were identified by nad1 and cox1 gene analysis, respectively. The haplotype diversity index was higher by cox1 gene analysis (0.547) in G1 strains compared with nad1 (0.433). The G1 genotype was the most pre...Continue Reading

References

Sep 1, 1992·Molecular and Biochemical Parasitology·J BowlesD P McManus
Dec 29, 1998·Clinical Radiology·D B Lewall
Oct 16, 2002·Trends in Parasitology·R C Andrew Thompson, Donald P McManus
Dec 12, 2003·Bioinformatics·Julio RozasRicardo Rozas
Mar 27, 2004·Parasitology·D P McManus, R C A Thompson
Jun 10, 2008·Experimental Parasitology·R C A Thompson
Feb 9, 2010·Veterinary Parasitology·R A OmerA Adam
Jun 16, 2010·Parasitology Research·Eshrat Bigom KiaHossein Mirhendi
Nov 24, 2011·Clinical & Developmental Immunology·Alessandra SiracusanoElena Ortona
Jul 31, 2013·International Journal for Parasitology·S M SadjjadiE B Kia
Aug 21, 2013·Acta Tropica·David Carmena, Guillermo A Cardona
Nov 26, 2013·International Journal for Parasitology·Cristian A Alvarez RojasMarshall W Lightowlers
Dec 24, 2014·The American Journal of Tropical Medicine and Hygiene·Sima RostamiMajid Fasihi Harandi
Nov 27, 2015·Tropical Medicine & International Health : TM & IH·Marcela Alejandra CucherLaura Kamenetzky
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Apr 6, 2016·Journal of Clinical and Translational Hepatology·Tina PakalaGeorge Y Wu
Jun 3, 2016·Revista Do Instituto De Medicina Tropical De São Paulo·Mitra SharbatkhoriMajid Fasihi Harandi
Oct 1, 2014·International Journal of Stroke : Official Journal of the International Stroke Society·Francesca Romana PezzellaUNKNOWN Women Stroke Association
Jan 31, 2017·Advances in Parasitology·J Eckert, R C A Thompson
Jan 31, 2017·Advances in Parasitology·K Brehm, U Koziol
Jan 31, 2017·Advances in Parasitology·R C A Thompson

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Citations

Mar 7, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Naunain MehmoodZafar Iqbal Khan
Jun 3, 2021·Clinical Microbiology Reviews·Mehdi BorhaniIraj Mobedi
Jul 16, 2021·Acta Parasitologica·Carlos ManterolaNayely García-Méndez

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