Genetic characterization of extraintestinal Escherichia coli isolates from chicken, cow and swine

AMB Express
Li ChenChengming Wang

Abstract

Phenotypic determination of antimicrobial resistance in bacteria is very important for diagnosis and treatment, but sometimes this procedure needs further genetic evaluation. Whole-genome sequencing plays a critical role in deciphering and advancing our understanding of bacterial evolution, transmission, and surveillance of antimicrobial resistance. In this study, whole-genome sequencing was performed on nineteen clinically extraintestinal Escherichia coli isolates from chicken, cows and swine and showing different antimicrobial susceptibility. A total of 44 different genes conferring resistance to 11 classes of antimicrobials were detected in 15 of 19 E. coli isolates (78.9%), and 22 types of plasmids were detected in 15/19 (78.9%) isolates. In addition, whole-genome sequencing of these 19 isolates identified 111 potential virulence factors, and 53 of these VFDB-annotated genes were carried by all these 19 isolates. Twelve different virulence genes were identified while the most frequent ones were gad (glutamate decarboxylase), iss (increased serum survival) and lpfA (long polar fimbriae). All isolates harbored at least one of the virulence genes. The findings from comparative genomic analyses of the 19 diverse E. coli isolate...Continue Reading

References

Jun 1, 1997·Journal of Bacteriology·S R Kim, T Komano
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Sep 3, 2005·Nature Reviews. Microbiology·Laura S FrostAriane Toussaint
Sep 8, 2005·Nature Reviews. Microbiology·Barth F Smets, Tamar Barkay
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Katie L HopkinsAlessandra Carattoli
Dec 13, 2006·International Journal of Systematic and Evolutionary Microbiology·Marco VenturaDouwe van Sinderen
Mar 25, 2009·Antimicrobial Agents and Chemotherapy·Alessandra Carattoli
Dec 8, 2009·Nature Reviews. Microbiology·Matthew A Croxen, B Brett Finlay
Feb 17, 2010·Nature Reviews. Microbiology·Olivier TenaillonErick Denamur
Feb 18, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Olivier ClermontErick Denamur
Jan 13, 2012·Journal of Clinical Microbiology·Mette V LarsenOle Lund
Apr 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carsten KrögerJay C D Hinton
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Feb 28, 2014·Journal of Clinical Microbiology·Katrine Grimstrup JoensenFrank M Aarestrup
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicola K PettyScott A Beatson
Apr 9, 2014·Trends in Plant Science·Jayson Gutiérrez, Steven Maere
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Nov 18, 2014·Nature Methods·Benjamin BuchfinkDaniel H Huson
Nov 19, 2015·Nucleic Acids Research·Lihong ChenQi Jin
Jun 22, 2016·Antimicrobial Agents and Chemotherapy·Kelson ZawackYrjö T Gröhn
Jul 7, 2016·Antimicrobial Agents and Chemotherapy·Patrick F McDermottShaohua Zhao

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Citations

Jul 31, 2019·Applied Microbiology and Biotechnology·Mingxu ZhouYu Lu
Jan 21, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rosa Elena Hernández-FillorVincent Perreten

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Datasets Mentioned

BETA
CP009072

Methods Mentioned

BETA
Whole Genome Sequencing
PCR

Software Mentioned

PhyML
WGS
CGE ResFinder
DIAMOND
CGE
MUSCLE
PlasmidFinder
CLC Genomics Workbench
Virulence Finder

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.