Genetic characterization of multidrug-resistant Mycobacterium bovis strains from a hospital outbreak involving human immunodeficiency virus-positive patients.

Journal of Clinical Microbiology
J BlázquezE Gómez-Mampaso

Abstract

Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they were M. bovis. All of the isolates were resistant to isoniazid, rifampin, pyrazinamide, ethambutol, streptomycin, para-aminosalicylic acid, clarithromycin, cycloserine, ethionamide, ofloxacin, capreomycin, and amikacin. By performing the standardized IS6110 fingerprinting by restriction fragment length polymorphism (RFLP) analysis, we were able to differentiate two groups (groups A and B) containing two (16 isolates) and three (3 isolates) IS6110 copies, respectively. These strains were typed by spoligotyping, developed to distinguish M. bovis strains and also to distinguish them from M. tuberculosis strains (J. Kamerbeek et al., J. Clin. Microbiol. 35:907-914, 1997). All the strains were confirmed to be M. bovis. In addition, spoligotyping showed a difference in only 1 of 43 spacers between RFLP groups A and B. The rpo beta region of several strains representative of each identified group was cloned and sequenced,...Continue Reading

References

May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Oct 1, 1990·Journal of Clinical Microbiology·U SjöbringH Miörner

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Citations

Nov 20, 1998·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M S ZaniniC E Salas
Apr 29, 2006·Journal of Veterinary Science·J P SinghP Chaudhuri
Dec 1, 2006·Annals of the New York Academy of Sciences·Eric EtterFrançois Roger
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·R LongA Fanning
Aug 7, 2010·Journal of Medical Microbiology·Shaukat BilalJoan Power
Oct 12, 1999·Microbiology·M J ZumárragaM I Romano
Oct 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jesús Blázquez
May 4, 2010·European Journal of Pediatrics·Chrysoula MaragouMaria N Tsolia
Jul 20, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G TudóUNKNOWN Tuberculosis Investigation Unit of Barcelona, Spain
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Cinzia MarianelliVincenzo Di Marco
Mar 20, 2012·Medical Microbiology and Immunology·Onno AkkermanAdri van der Zanden
Jan 31, 2019·Epidemiologic Reviews·Rodrigo Macedo CoutoEliseu Alves Waldman
Jan 18, 2005·Memórias do Instituto Oswaldo Cruz·Patrícia M ParreirasPhilip N Suffys
Jan 15, 2015·Plastic and Reconstructive Surgery. Global Open·Mehmet Derviş GünerŞadan Ay
Jun 9, 2017·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Gerard de VriesSytze T Keizer
Jun 21, 2019·Microorganisms·Rachael A FiewegerBrian C VanderVen
Dec 13, 2018·Frontiers in Microbiology·Daniela BritesSebastien Gagneux
Apr 17, 2007·Lancet·Charles O Thoen, Philip A LoBue
Mar 8, 2005·Journal of Clinical Microbiology·S SamperUNKNOWN Spanish Working Group on MDR-TB
Sep 22, 2006·Journal of Clinical Microbiology·Nicoletta LariCarlo Garzelli
Sep 13, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J EstebanM L Fernández Guerrero

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