Abstract
Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they were M. bovis. All of the isolates were resistant to isoniazid, rifampin, pyrazinamide, ethambutol, streptomycin, para-aminosalicylic acid, clarithromycin, cycloserine, ethionamide, ofloxacin, capreomycin, and amikacin. By performing the standardized IS6110 fingerprinting by restriction fragment length polymorphism (RFLP) analysis, we were able to differentiate two groups (groups A and B) containing two (16 isolates) and three (3 isolates) IS6110 copies, respectively. These strains were typed by spoligotyping, developed to distinguish M. bovis strains and also to distinguish them from M. tuberculosis strains (J. Kamerbeek et al., J. Clin. Microbiol. 35:907-914, 1997). All the strains were confirmed to be M. bovis. In addition, spoligotyping showed a difference in only 1 of 43 spacers between RFLP groups A and B. The rpo beta region of several strains representative of each identified group was cloned and sequenced,...Continue Reading
References
Jun 1, 1991·Journal of Clinical Microbiology·I OtalB Gicquel
May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Oct 1, 1990·Journal of Clinical Microbiology·U SjöbringH Miörner
Aug 1, 1993·Journal of Clinical Microbiology·D van SoolingenJ D van Embden
Oct 1, 1994·Journal of Clinical Microbiology·D van SoolingenJ D van Embden
Dec 1, 1993·Molecular Microbiology·P M GroenenJ D van Embden
Mar 13, 1993·Lancet·A TelentiT Bodmer
Oct 1, 1993·Antimicrobial Agents and Chemotherapy·A TelentiT Bodmer
Nov 1, 1993·AIDS·E BouvetF Vachon
Feb 1, 1993·Journal of Clinical Microbiology·J D van EmbdenT M Shinnick
Nov 1, 1995·Journal of Clinical Microbiology·M GutiérrezC Martín
Apr 1, 1997·Journal of Clinical Microbiology·J KamerbeekJ van Embden
Citations
Nov 20, 1998·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M S ZaniniC E Salas
May 17, 2001·Antimicrobial Agents and Chemotherapy·L A SechiG Fadda
Jun 3, 2006·Journal of Bacteriology·Richard C HuardJohn L Ho
Apr 29, 2006·Journal of Veterinary Science·J P SinghP Chaudhuri
Dec 1, 2006·Annals of the New York Academy of Sciences·Eric EtterFrançois Roger
Jul 6, 2014·BioMed Research International·Victoria O AdetunjiJinru Chen
Feb 13, 2001·Journal of Internal Medicine·D Van Soolingen
Jan 20, 1999·Molecular Pathology : MP·A G van der ZandenJ D van Embden
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·R LongA Fanning
Aug 7, 2010·Journal of Medical Microbiology·Shaukat BilalJoan Power
Oct 12, 1999·Microbiology·M J ZumárragaM I Romano
Apr 13, 2000·Journal of Bacteriology·J D van EmbdenL M Schouls
Oct 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jesús Blázquez
May 4, 2010·European Journal of Pediatrics·Chrysoula MaragouMaria N Tsolia
Jul 20, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G TudóUNKNOWN Tuberculosis Investigation Unit of Barcelona, Spain
Oct 5, 2002·Clinical Microbiology Reviews·José L Martínez, Fernando Baquero
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Cinzia MarianelliVincenzo Di Marco
Mar 20, 2012·Medical Microbiology and Immunology·Onno AkkermanAdri van der Zanden
Jan 31, 2019·Epidemiologic Reviews·Rodrigo Macedo CoutoEliseu Alves Waldman
Jan 18, 2005·Memórias do Instituto Oswaldo Cruz·Patrícia M ParreirasPhilip N Suffys
Jan 15, 2015·Plastic and Reconstructive Surgery. Global Open·Mehmet Derviş GünerŞadan Ay
Apr 22, 2009·Scandinavian Journal of Infectious Diseases·Rahel IwnetuAbraham Aseffa
Apr 2, 1999·Applied and Environmental Microbiology·L A SechiS Zanetti
Jun 9, 2017·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Gerard de VriesSytze T Keizer
Jun 21, 2019·Microorganisms·Rachael A FiewegerBrian C VanderVen
Dec 13, 2018·Frontiers in Microbiology·Daniela BritesSebastien Gagneux
Apr 17, 2007·Lancet·Charles O Thoen, Philip A LoBue
Mar 8, 2005·Journal of Clinical Microbiology·S SamperUNKNOWN Spanish Working Group on MDR-TB
Jul 5, 2003·Journal of Clinical Microbiology·Tanja KubicaStefan Niemann
Dec 21, 2007·Journal of Clinical Microbiology·Beatriz RomeroLucas Domínguez
Sep 22, 2006·Journal of Clinical Microbiology·Nicoletta LariCarlo Garzelli
Feb 12, 1998·Journal of Clinical Microbiology·L E Espinosa de los MonterosJ Blázquez
Mar 13, 1999·Journal of Clinical Microbiology·M C GutiérrezV Vincent
Jan 16, 1999·Journal of Clinical Microbiology·M J ZumárragaM I Romano
May 5, 2006·Journal of Clinical Microbiology·Heekyung ParkChulhun L Chang
Sep 7, 2006·Journal of Clinical Microbiology·Beatriz RomeroLucas Domínguez
Feb 3, 2000·Journal of Clinical Microbiology·H SoiniJ M Musser
Sep 13, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J EstebanM L Fernández Guerrero