PMID: 8606175Apr 1, 1996Paper

Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus" C70

Journal of Bacteriology
C Le MarrecA Trautwetter

Abstract

All the essential genetic determinants for site-specific integration of corynephage phi AAU2 are contained within a 1,756-bp DNA fragment, carried on the integrative plasmid p5510, and are shown to be functional in Escherichia coli. One open reading frame, ORF4, encoding a protein of 266 amino acids was shown to represent the phi AAU2 integrase. The nucleotide sequence of the phi AAU2 attachment site, attP, and the attB, attL, and attR sequences in the host "Arthrobacter aureus" C70 were determined. Identical nucleotide sequences were shown to be responsible for the integration of p5510 in the chromosomes of Corynebacterium glutamicum, Brevibacterium divaricatum, and B. lactofermentum, and a sequence almost identical to attB was found to be present in these three strains. In contrast to other phage site-specific recombination systems, a plasmid encompassing only int-attP failed to integrate into the host chromosome. This led to the identification of an 800-bp noncoding region, immediately upstream of int, absolutely required for site-specific integration of p5510.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1992·Journal of Bacteriology·A M Campbell
Oct 11, 1991·Nucleic Acids Research·H Rausch, M Lehmann
Sep 1, 1991·Journal of General Microbiology·A Trautwetter, C Blanco
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1990·Journal of General Microbiology·S BonnassieA M Sicard
Mar 1, 1990·Journal of General Microbiology·H SonnenH J Kutzner
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C Y Lee, J J Iandolo
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Jun 1, 1969·Journal of Virology·R K Holmes, L Barksdale
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·K MizuuchiA Landy
Jul 1, 1995·Research in Microbiology·S MoreauA Trautwetter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.