PMID: 9446910Feb 3, 1998Paper

Genetic comparison between Spirometra erinacei and S. mansonoides using PCR-RFLP analysis

The Korean Journal of Parasitology
S U LeeC K Phares

Abstract

The only observed morphological difference between Spirometra erinacei and S. mansonoides is the uterine shape of the mature proglottid. Two species of worms are thought to be evolutionarily closely related. Biomolecular comparison of the two worms by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to observe the genetic distance. The 28s rDNA, mitochondrial cytochrome c oxidase subunit I (mCOI), and ribosomal internal transcribed spacer 1 (ITS1) fragments were obtained from the worms by PCR. The PCR products were cleaved by 5 four-base pair restriction enzyme combinations (Msp I, Hae III, Alu I, Cfo I, Rsa I), electrophoresed and analyzed with PAUP 3.1.1. The fragment patterns of 28S rDNA and ITS1 demonstrated that two worms were in identical systematic tree with bootstrap number 94 and 100, respectively. As for mCOI, bootstrap number was 74 in a different tree. Above results are indicative of recent common ancestry between S. erinacei and S. mansonoides.

References

Feb 1, 1993·Molecular and Biochemical Parasitology·J Bowles, D P McManus
Jan 1, 1993·Methods in Enzymology·B M BowditchM J Braun
Jun 1, 1984·Kisaengch'unghak chapchi. The Korean journal of parasitology·Soon Hyung LeeSeung Yull Cho

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Citations

Sep 14, 2015·The Lancet Infectious Diseases·Quan LiuXing-Quan Zhu
May 29, 2016·Parasitology International·Gregório Guilherme AlmeidaHudson Alves Pinto
Sep 24, 2016·The Korean Journal of Parasitology·Hyeong-Kyu JeonKeeseon S Eom
Aug 3, 2016·The American Journal of Tropical Medicine and Hygiene·Nicholas KavanaYee Ling Lau

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