Genetic composition of replication competent clonal HIV-1 variants isolated from peripheral blood mononuclear cells (PBMC), HIV-1 proviral DNA from PBMC and HIV-1 RNA in serum in the course of HIV-1 infection

Virology
Diana Edo-MatasHanneke Schuitemaker

Abstract

The HIV-1 quasispecies in peripheral blood mononuclear cells (PBMC) is considered to be a mix of actively replicating, latent, and archived viruses and may be genetically distinct from HIV-1 variants in plasma that are considered to be recently produced. Here we analyzed the genetic relationship between gp160 env sequences from replication competent clonal HIV-1 variants that were isolated from PBMC and from contemporaneous HIV-1 RNA in serum and HIV-1 proviral DNA in PBMC of four longitudinally studied therapy naïve HIV-1 infected individuals. Replication competent clonal HIV-1 variants, HIV-1 RNA from serum, and HIV-1 proviral DNA from PBMC formed a single virus population at most time points analyzed. However, an under-representation in serum of HIV-1 sequences with predicted CXCR4 usage was sometimes observed implying that the analysis of viral sequences from different sources may provide a more complete assessment of the viral quasispecies in peripheral blood in vivo.

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Citations

May 29, 2014·Scientific Reports·Esther F GijsbersNeeltje A Kootstra
Jun 25, 2013·Virology·Lindsay M EyzaguirreMohammad M Sajadi
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lina JosefssonSarah E Palmer
Feb 10, 2011·Journal of Translational Medicine·Hanneke SchuitemakerPaolo Lusso

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