Abstract
The humoral response of twelve strains of mice to immunization with either normal adult human haemoglobin (HbA1) or sickle-cell haemoglobin (HbS) was studied using a quantitative enzyme-linked immunosorbant assay (ELISA). Results indicate the presence of at least two genes that control the magnitude of this response, one of which is located at the 'D-end' of the H-2 complex. H-2 haplotypes s, q and k are associated with a low response, haplotypes d and a with a high response, and the b haplotype with an intermediate response. No difference in the pattern or magnitude of the response was observed when HbS was used as the immunogen in place of HbA1. The N-terminal octapeptides of the beta chains of HbA1 and HbS were synthesized and the presence of antibodies specific for the N-terminal octapeptides was tested. The magnitude of the anti-N-terminal response correlated with that to the intact molecules with one exception. A much lower response to the beta s-peptide was found in the C57BL/10Sn mice. Monoclonal antibodies were prepared to HbA1 and HbS. Three clones were studied for their reaction with HbA1, HbS, and the beta A1 and beta S peptides. One clone was found which binds both the beta A1 and beta S peptides. This indicates th...Continue Reading
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