Genetic damage, but limited evidence of oxidative stress markers in diethyl maleate-induced glutathione depleted mouse lymphoma L5178Y (TK(+/-)) cell cultures

Toxicology Mechanisms and Methods
David R GeterB Bhaskar Gollapudi

Abstract

Depletion of glutathione (GSH) in cells exposed to certain xenobiotics has been proposed to result in oxidative stress, which could lead to damage of cellular macromolecules such as proteins, lipids, and DNA. Diethyl maleate (DEM) is known to conjugate with GSH and rapidly lower cellular GSH levels. The objective of this study was to investigate the influence of DEM-induced GSH depletion on various genotoxicity and gene expression end points in mouse lymphoma L5178Y (TK(+/-)) cell cultures. Cells were exposed to DEM for 4 h at concentrations of 0, 6.7, 13.5, 26.9, 53.8, 107.6, 215.3, and 430.6 µg/mL (0.039-2.5 mM). Genotoxicity was evaluated by examining the induction of in vitro micronuclei (20 h post-treatment) and DNA strand breaks as measured by comet (immediately following treatment), and correlating these observations to cellular GSH levels. In the current study, GSH was decreased more than 50% at the lowest test concentration (6.7 µg/mL) and more than 95% at ≥ 107.6 µg/mL. A significant increase in micronuclei and DNA strand breaks was observed at concentrations of ≥ 26.9 µg/mL. Gene expression of seven apoptosis and oxidative-stress related genes showed significant alterations in only three genes only at the highest tes...Continue Reading

References

Jul 1, 1979·Acta Pharmacologica Et Toxicologica·I AnundiA H Stead
May 1, 1988·Mutagenesis·J Wangenheim, G Bolcsfoldi
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jul 1, 1967·The Biochemical Journal·E Boyland, L F Chasseaud
Mar 15, 1981·Chemico-biological Interactions·M Younes, C P Siegers
Aug 1, 1996·Surgery·R W WatsonJ C Marshall
Jan 1, 1996·Cancer Treatment and Research·A Raha, K D Tew
Jul 1, 1997·The American Journal of Physiology·B S Dixon, M J Dennis
Aug 1, 1997·Mutation Research·M Kirsch-Volders
Jan 22, 1998·Mutation Research·P G WellsL M Winn
Apr 29, 1998·Annual Review of Physiology·G KroemerM Resche-Rigon
Jan 30, 1999·The International Journal of Biochemistry & Cell Biology·M MadeshK A Balasubramanian
Jan 29, 2000·Mutation Research·P VillaniE Cordelli
Feb 24, 2001·Antioxidants & Redox Signaling·M A TirmensteinM W Fariss
May 15, 2001·Regulatory Toxicology and Pharmacology : RTP·M W Pariza, E A Johnson
Jul 11, 2002·Free Radical Biology & Medicine·Fernando Cardozo-PelaezJuan R Sánchez-Ramos
Dec 11, 2002·Mutagenesis·A HartmannUNKNOWN 4th International Comet Assay Workshop
Oct 17, 2003·The Journal of Physiology·Julio F Turrens
May 12, 2005·International Journal of Pediatric Otorhinolaryngology·Mustafa CemekKöksal Yuca
Jul 1, 2005·Sleep & Breathing = Schlaf & Atmung·Mohammed A Alzoghaibi, Ahmed S O Bahammam
Jul 15, 2005·European Journal of Clinical Nutrition·R VijayaraghavanC Panneerselvam
Aug 12, 2005·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Henrik E Poulsen
Mar 1, 2006·BMC Bioinformatics·Joshua S YuanC Neal Stewart
Jul 26, 2006·Free Radical Biology & Medicine·Guillermo Esteban-Pretel, M Pilar López-García
Aug 15, 2006·The International Journal of Biochemistry & Cell Biology·A BonfigliF Amicarelli
Nov 28, 2006·DNA Repair·Arne Klungland, Svein Bjelland
Jan 20, 2007·Mutagenesis·Janne Kjaersgaard FolkmannPeter Møller
Jun 16, 2007·Life Sciences·Subir Kumar Das, D M Vasudevan
Aug 19, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liping JiangLaifu Zhong
Dec 7, 2007·Mutation Research·Andrew R Collins

❮ Previous
Next ❯

Citations

Jun 13, 2013·Journal of Agricultural and Food Chemistry·Hisham R IbrahimTakeshi Miyata

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Chemistry & Biodiversity
Robert S SorokanichJames C Dabrowiak
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Gary M Williams
© 2021 Meta ULC. All rights reserved