Genetic deletion of keratin 8 corrects the altered bone formation and osteopenia in a mouse model of cystic fibrosis

Human Molecular Genetics
Carole Le HenaffPierre Marie

Abstract

Patients with cystic fibrosis (CF) display low bone mass and alterations in bone formation. Mice carrying the F508del genetic mutation in the cystic fibrosis conductance regulator (Cftr) gene display reduced bone formation and decreased bone mass. However, the underlying molecular mechanisms leading to these skeletal defects are unknown, which precludes the development of an efficient anti-osteoporotic therapeutic strategy. Here we report a key role for the intermediate filament protein keratin 8 (Krt8), in the osteoblast dysfunctions in F508del-Cftr mice. We found that murine and human osteoblasts express Cftr and Krt8 at low levels. Genetic studies showed that Krt8 deletion (Krt8(-/-)) in F508del-Cftr mice increased the levels of circulating markers of bone formation, corrected the expression of osteoblast phenotypic genes, promoted trabecular bone formation and improved bone mass and microarchitecture. Mechanistically, Krt8 deletion in F508del-Cftr mice corrected overactive NF-κB signaling and decreased Wnt-β-catenin signaling induced by the F508del-Cftr mutation in osteoblasts. In vitro, treatment with compound 407, which specifically disrupts the Krt8-F508del-Cftr interaction in epithelial cells, corrected the abnormal NF-...Continue Reading

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Citations

Nov 12, 2016·Cell Death and Differentiation·Zhenqing LiuHsiao-Chang Chan
Jan 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marco RusnatiPasqualina D'Ursi
Sep 9, 2016·American Journal of Physiology. Cell Physiology·Megan C MoorerRobert J Bloch
May 25, 2021·Frontiers in Cell and Developmental Biology·Claire DumortierDenise Al Alam

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