Genetic determinants of mouse hepatitis virus strain 1 pneumovirulence.

Journal of Virology
Julian LeibowitzSusan R Weiss

Abstract

We report here investigation into the genetic basis of mouse hepatitis virus strain 1 (MHV-1) pneumovirulence. Sequencing of the 3' one-third of the MHV-1 genome demonstrated that the genetic organization of MHV-1 was similar to that of other strains of MHV. The hemagglutinin esterase (HE) protein was truncated, and reverse transcription-PCR (RT-PCR) studies confirmed previous work that suggested that the MHV-1 HE is a pseudogene. Targeted recombination was used to select chimeric viruses containing either the MHV-1 S gene or genes encoding all of the MHV-1 structural proteins, on an MHV-A59 background. Challenge studies in mice demonstrated that expression of the MHV-1 S gene within the MHV-A59 background (rA59/S(MHV-1)) increased the pneumovirulence of MHV-A59, and mice infected with this recombinant virus developed pulmonary lesions that were similar to those observed with MHV-1, although rA59/S(MHV-1) was significantly less virulent. Chimeras containing all of the MHV-1 structural genes on an MHV-A59 background were able to reproduce the severe acute respiratory syndrome (SARS)-like pathology observed with MHV-1 and reproducibly increased pneumovirulence relative to rA59/S(MHV-1), but were still much less virulent than MHV-...Continue Reading

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Citations

Sep 26, 2014·The Journal of General Virology·Brenna McGruder, Julian L Leibowitz
Dec 31, 2014·Virologica Sinica·Zhangsheng YangHong Tang
Jun 27, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Bing HanMingyao Liu
Dec 17, 2020·Animals : an Open Access Journal From MDPI·Valentina ZappulliMassimo Castagnaro
Mar 10, 2021·European Journal of Immunology·Sarah GrabherrNatalia Barbara Pikor

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