Jul 14, 1999

Genetic determinants of plasma ACE and renin activity in young normotensive twins

Journal of Hypertension
G P RossiA C Pessina

Abstract

We investigated the determinants of plasma renin activity (PRA) and plasma levels of angiotensin-converting enzyme (pACE), including the effect of the D/I polymorphism of the angiotensin-converting enzyme (ACE) gene, in monozygotic (MZ) and dizygotic (DZ) twins. Sixty-nine pairs of twins underwent measurements of blood pressure, pACE and ACE D/I genotyping. In addition, in 30 pairs ambulatory blood pressure (ABP) monitoring was carried out. To ascertain twin's zygosity, some highly discriminating variable number of tandem repeats micro- and mini-satellite systems were analysed by polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis and silver staining. The D/I polymorphism was assessed by PCR; pACE was measured in triplicate with a colorimetric assay, and PRA by a commercial kit. In DZ twins, identity by descent of the D/I alleles was examined by PCR amplification of a highly polymorphic simple sequence repeat at the human growth hormone gene. pACE levels were significantly (P < 0.01) higher in DD (9.27 +/- 2.60 IU/l, mean +/-SD) than in II (6.68 +/- 3.0), with DI having intermediate levels (7.93 +/- 2.7). No difference of PRA between different D/I genotypes was found. Twin data analysis showed a stati...Continue Reading

  • References11
  • Citations13

References

  • References11
  • Citations13

Citations

Mentioned in this Paper

Variable Number of Tandem Repeats
Short Tandem Repeat
Quantitative Trait Loci
Growth Hormone Activity
Dysequilibrium Syndrome
Monozygotic Twins
Minisatellite Repeats
Gene Amplification Technique
Growth Hormone Measurement
Blood Pressure

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