PMID: 9420260Jan 7, 1998Paper

Genetic determinants of Rous sarcoma virus particle size

Journal of Virology
N K KrishnaJ W Wills

Abstract

The Gag proteins of retroviruses are the only viral products required for the release of membrane-enclosed particles by budding from the host cell. Particles released when these proteins are expressed alone are identical to authentic virions in their rates of budding, proteolytic processing, and core morphology, as well as density and size. We have previously mapped three very small, modular regions of the Rous sarcoma virus (RSV) Gag protein that are necessary for budding. These assembly domains constitute only 20% of RSV Gag, and alterations within them block or severely impair particle formation. Regions outside of these domains can be deleted without any effect on the density of the particles that are released. However, since density and size are independent parameters for retroviral particles, we employed rate-zonal gradients and electron microscopy in an exhaustive study of mutants lacking the various dispensable segments of Gag to determine which regions would be required to constrain or define the particle dimensions. The only sequence found to be absolutely critical for determining particle size was that of the initial capsid cleavage product, CA-SP, which contains all of the CA sequence plus the spacer peptides locate...Continue Reading

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Citations

Oct 22, 2002·Journal of Virology·Marc C JohnsonVolker M Vogt
Jul 5, 2005·Journal of Virology·Lisa Z ScheifeleLeslie J Parent
Mar 7, 2014·Journal of Virology·Di L BushVolker M Vogt
Apr 11, 2014·Journal of Virology·Matthew R EnglandRebecca C Craven
Dec 19, 2000·Journal of Virology·T M Cairns, R C Craven
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lisa Z ScheifeleLeslie J Parent
Dec 13, 2002·Journal of Virology·Lisa Z ScheifeleLeslie J Parent
Nov 6, 2007·Journal of Virology·Scott P KenneyLeslie J Parent
Sep 16, 2004·Journal of Virology·Jared L SpidelJohn W Wills
Jan 29, 2011·Journal of Virology·Kristina ClemensSuzanne Sandmeyer
Oct 3, 2014·Journal of Virology·Karolína Strohalmová-BohmováMichaela Rumlová
Feb 18, 2011·Journal of Virology·Siddhartha A K DattaAlan Rein
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Stanislaw J KaczmarczykDeb K Chatterjee
Nov 12, 2020·Journal of Virology·Ritesh TandonRobert J Linhardt
Feb 11, 1999·Journal of Virology·L GarnierJ W Wills
Feb 28, 2002·Journal of Molecular Biology·Keith MayoEric Barklis
Jun 30, 2000·Structure·R L KingstonM G Rossmann

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