Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish.

Proceedings of the National Academy of Sciences of the United States of America
Kazuhide AsakawaKoichi Kawakami

Abstract

Targeted gene expression is a powerful approach to study the function of genes and cells in vivo. In Drosophila, the P element-mediated Gal4-UAS method has been successfully used for this purpose. However, similar methods have not been established in vertebrates. Here we report the development of a targeted gene expression methodology in zebrafish based on the Tol2 transposable element and its application to the functional study of neural circuits. First, we developed gene trap and enhancer trap constructs carrying an engineered yeast Gal4 transcription activator (Gal4FF) and transgenic reporter fish carrying the GFP or the RFP gene downstream of the Gal4 recognition sequence (UAS) and showed that the Gal4FF can activate transcription through UAS in zebrafish. Second, by using this Gal4FF-UAS system, we performed large-scale screens and generated a large collection of fish lines that expressed Gal4FF in specific tissues, cells, and organs. Finally, we developed transgenic effector fish carrying the tetanus toxin light chain (TeTxLC) gene downstream of UAS, which is known to block synaptic transmission. We crossed the Gal4FF fish with the UAS:TeTxLC fish and analyzed double transgenic embryos for defects in touch response. From ...Continue Reading

References

May 1, 1990·Neuron·B TrevarrowC B Kimmel
Oct 6, 1988·Nature·I SadowskiM Ptashne
Aug 25, 1988·Nature·G Gill, M Ptashne
Dec 19, 1998·Journal of Neurobiology·L Saint-Amant, P Drapeau
Mar 12, 1999·Mechanisms of Development·N Scheer, J A Campos-Ortega
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·K KawakamiN Kawakami
May 5, 2001·Developmental Biology·R W Köster, S E Fraser
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Jun 25, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shin-ichi HigashijimaJoseph R Fetcho
Jul 9, 2004·Developmental Cell·Koichi KawakamiMasayoshi Mishina
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kimberly J HarmsAnn Marie Craig
Aug 20, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Koichi Kawakami
Feb 14, 2006·Journal of Neurobiology·Gerald B Downes, Michael Granato
May 26, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yukiko KimuraShin-ichi Higashijima
Mar 21, 2007·Nature Methods·Ethan K ScottHerwig Baier

❮ Previous
Next ❯

Citations

Feb 18, 2010·Brain Structure & Function·Jonathan J SagerEdward A Burton
Feb 18, 2009·Molecular Genetics and Genomics : MGG·Lisette A MaddisonWenbiao Chen
Jan 27, 2011·Neurogenetics·Sabine L RenningerRalf Dahm
Mar 29, 2013·Marine Biotechnology·Feng XiongYong-Hua Sun
Jul 4, 2013·Chemical Reviews·Sandeep Basu, Chetana Sachidanandan
May 21, 2013·Nature Neuroscience·Norihito KishimotoKazunobu Sawamoto
Dec 3, 2011·Nature Protocols·Maximiliano L SusterKoichi Kawakami
Dec 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Akihiro UrasakiKoichi Kawakami
Jun 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya KoideYoshihiro Yoshihara
Jul 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Martin DistelReinhard W Köster
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Aristides B ArrenbergHerwig Baier
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Akira MutoKoichi Kawakami
Sep 2, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ryohei YagiKonrad Basler
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hironori WadaAlain Ghysen
Jun 17, 2008·Zebrafish·Marnie E HalpernSteven D Leach
Nov 4, 2008·Briefings in Functional Genomics & Proteomics·Li-En JaoShawn M Burgess
Nov 13, 2012·Nucleic Acids Research·Peng HuangBo Zhang
Apr 24, 2008·Development, Growth & Differentiation·Wataru Shoji, Mika Sato-Maeda
May 17, 2008·Development, Growth & Differentiation·Kazuhide Asakawa, Koichi Kawakami
Feb 5, 2010·Genome Génome / Conseil National De Recherches Canada·Ruth B Phillips, Robert H Devlin
Oct 19, 2010·BMC Developmental Biology·Koichi KawakamiMikio Yoshida
Sep 10, 2009·BMC Genomics·Igor KondrychynVladimir Korzh
Oct 17, 2009·BMC Genomics·Maximiliano L SusterKoichi Kawakami
Sep 17, 2013·BMC Genomics·Jorune BalciunieneDarius Balciunas
Aug 29, 2009·BMC Neuroscience·Xiao-Feng ZhaoAnders Fjose
May 2, 2013·Development·Sebastian S GeretyDavid G Wilkinson
Oct 27, 2011·Disease Models & Mechanisms·Norihito KishimotoKazunobu Sawamoto
Feb 28, 2009·PloS One·Stefan HansMichael Brand
Feb 8, 2011·PloS One·Adèle Faucherre, Hernán López-Schier

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Martin DistelReinhard W Köster
Developmental Dynamics : an Official Publication of the American Association of Anatomists
Eri OguraYusuke Kamachi
© 2021 Meta ULC. All rights reserved