PMID: 9551177Jan 1, 1997Paper

Genetic dissection of vertebrate processes in the zebrafish: a comparison of uniparental and two-generation screens

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
K C Cheng, J L Moore

Abstract

Genetic dissection is used to identify important genes in biological processes and is accomplished through the generation and study of mutations in model organisms. In diploid organisms, recessive mutations must be rendered hemizygous or homozygous for the mutant phenotype to be detected. In zebrafish (Danio rerio), this can be accomplished in two ways: (i) crosses between siblings who are potential carriers (in two-generation screens) and (ii) the generation of uniparental progeny. Two-generation screens have been the most productive to date but require substantial resources. However, uniparental screens involving haploids and half-tetrads (products of meiosis I) have also been productive, and require more modest resources. In genetic screens, the fraction of an average genome that is heterozygous (heterozygosity index) is inversely proportional to the likelihood that separate recessive mutations will be homozygous at the same time. Heterozygosity indices for haploid, half-tetrad, and two-generation screens are 0, 66, and 87.5%, respectively. Family sizes required to minimize bias in half-tetrad screens are also calculated. We conclude that gynogenetic half-tetrad screens are genetically robust and technically accessible to th...Continue Reading

Citations

Dec 31, 2003·Experimental Gerontology·Glenn S Gerhard
May 28, 2004·Journal of Biochemical and Biophysical Methods·Martin A GrundyGlenn S Gerhard
Sep 3, 2003·FEMS Microbiology Letters·Michael G ProutyKarl E Klose
Sep 6, 2002·Experimental Gerontology·Glenn S GerhardKeith C Cheng
Jul 2, 2003·Nature Reviews. Cancer·Howard M Stern, Leonard I Zon
Jul 29, 2003·Aging Cell·Glenn S Gerhard, Keith C Cheng
Jan 7, 2005·Journal of Thrombosis and Haemostasis : JTH·P JagadeeswaranB Thattaliyath
Aug 5, 2006·Genetics·Jessica L MooreKeith C Cheng
Dec 31, 2011·Genome Medicine·Corey S MartinLeonard I Zon
Dec 1, 2004·Annual Review of Genetics·Elizabeth Pradel, Jonathan J Ewbank

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