Genetic Diversity and Drug Resistance of HIV-1 Circulating in North Sulawesi, Indonesia

AIDS Research and Human Retroviruses
Shuhei UedaMasanori Kameoka

Abstract

Manado, the capital city of North Sulawesi, is a unique region in Indonesia because of its religion. We collected peripheral blood samples from 63 individuals on antiretroviral therapy. The amplification of viral genomic fragments, viral subtyping, detection of HIV drug resistance-associated mutations (DRAMs), and phylogenetic analyses were performed. Viral subtyping revealed that the most prevalent HIV type 1 (HIV-1) subtype/circulating recombinant form (CRF) was CRF01_AE (84.1%), followed by subtype B (6.8%) and recombinants between CRF01_AE and CRF02_AG (4.5%). Although no major DRAMs were present in protease genes, they were detected in reverse transcriptase (RT) genes. Nine of 38 samples (23.7%) had major DRAMs against nucleoside RT inhibitors (NRTIs) and/or non-NRTIs. The results of phylogenetic analyses indicated that CRF01_AE in North Sulawesi is related to that in Bali. Therefore, Bali may play an important role in circulating CRF01_AE in North Sulawesi.

References

Mar 29, 2018·AIDS Research and Human Retroviruses·Siti Qamariyah KhairunisaMasanori Kameoka

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Citations

Aug 18, 2020·AIDS Research and Human Retroviruses·Siti Qamariyah KhairunisaMasanori Kameoka

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Methods Mentioned

BETA
PCR
RIP

Software Mentioned

BLAST
MEGA6
PhyML
TreeAnnotator
FigTree
Genetyx
Tracer
BEAST

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