Genetic diversity and evolutionary characteristics of type 2 porcine reproductive and respiratory syndrome virus in southeastern China from 2009 to 2014

Archives of Virology
Jian-Kui LiuMan-Lin Luo

Abstract

The objective of this study was to assess the genetic diversity of porcine reproductive and respiratory syndrome virus circulating in Fujian province (southeastern China). Based on 53 ORF5 nucleotide sequences collected from nine sites, both highly pathogenic (sublineage 8.7) and lineage 1 strains were circulating in Fujian in 2009-2014 along with lineages 3 and 5.1. Notably, the lineage 1 strains were closely related to the NADC30 strain circulating in North America and were the predominant strains in 2014. In addition, we found that nonstructural protein 2 (NSP2) was the most variable nonstructural protein in Fujian isolates, with a 36-amino-acid (aa) insertion and seven different deletions detected in the 53 sequences examined. Similarly, analysis of GP5 amino acid sequences showed that the isolates were highly variable in primary neutralizing epitopes. Interesting, FJ3.2 and FJ7-2 strains have the mutation N44K, but they exhibited high replication and high titers in MARC-145 and PAM cells. The complete genome sequences determined for 12 type 2 isolates were 82.1-99.3% identical and were 15,016-15,407 nucleotides (nt), in length excluding the poly(A) tail. The strains also shared 88.2-99.4% identity with strain VR2332 (the p...Continue Reading

References

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Citations

Mar 10, 2018·Transboundary and Emerging Diseases·H-M WangX-H Cai
Oct 29, 2020·Transboundary and Emerging Diseases·Jiankui LiuChunhua Wei
Dec 12, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jiankui LiuXiaoyan Yang

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Methods Mentioned

BETA
PCRs
PCR
glycosylation
Phylogenies

Software Mentioned

MEGA
CLUSTAL X
BootScan
BEAST
NetNGlyc
GeneConv
TreeAnnotator
SiScan
RDP
MaxChi

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