Genetic Diversity and Pathogenic Potential of Attaching and Effacing Escherichia coli O26:H11 Strains Recovered from Bovine Feces in the United States

Applied and Environmental Microbiology
Sarah A IsonPatrick Fach

Abstract

Escherichia coli O26 has been identified as the most common non-O157 Shiga toxin-producing E. coli (STEC) serogroup to cause human illnesses in the United States and has been implicated in outbreaks around the world. E. coli has high genomic plasticity, which facilitates the loss or acquisition of virulence genes. Attaching and effacing E. coli (AEEC) O26 strains have frequently been isolated from bovine feces, and there is a need to better characterize the relatedness of these strains to defined molecular pathotypes and to describe the extent of their genetic diversity. High-throughput real-time PCR was used to screen 178 E. coli O26 isolates from a single U.S. cattle feedlot, collected from May to July 2011, for the presence or absence of 25 O26 serogroup-specific and virulence-associated markers. The selected markers were capable of distinguishing these strains into molecularly defined groups (yielding 18 unique marker combinations). Analysis of the clustered regularly interspaced short palindromic repeat 1 (CRISPR1) and CRISPR2a loci further discriminated isolates into 24 CRISPR types. The combination of molecular markers and CRISPR typing provided 20.8% diversity. The recent CRISPR PCR target SP_O26-E, which was previously...Continue Reading

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Citations

Oct 10, 2015·Environmental Science and Pollution Research International·Sabine DelannoyPatrick Fach
Jul 9, 2017·Applied and Environmental Microbiology·Peter C H FengLothar Beutin
Jan 13, 2018·Pathogens and Disease·Liliana Medina-AparicioIsmael Hernández-Lucas
Nov 6, 2015·Frontiers in Cellular and Infection Microbiology·Alejandra KrügerJohn W A Rossen
Apr 20, 2021·International Journal of Microbiology·Indira T KudvaClint A Loest

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