Genetic Diversity and Phylogenetic Relationships of Coevolving Symbiont-Harboring Insect Trypanosomatids, and Their Neotropical Dispersal by Invader African Blowflies (Calliphoridae)

Frontiers in Microbiology
Tarcilla C BorghesanErney P Camargo

Abstract

This study is about the inter- and intra-specific genetic diversity of trypanosomatids of the genusAngomonas, and their association with Calliphoridae (blowflies) in Neotropical and Afrotropical regions. Microscopic examination of 3,900 flies of various families, mostly Calliphoridae, revealed that 31% of them harbored trypanosomatids. Small subunit rRNA (SSU rRNA) barcoding showed thatAngomonaspredominated (46%) over the other common trypanosomatids of blowflies of generaHerpetomonasandWallacemonas. AmongAngomonasspp.,A. deaneiwas much more common than the two-other species,A. desouzaiandA. ambiguus. Phylogenetic analyses based on SSU rRNA, glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) and internal transcribed spacer rDNA (ITS rDNA) sequences revealed a marked genetic diversity withinA. deanei, which comprised four infraspecific genotypes (Dea1-Dea4), and four corresponding symbiont genotypes (Kcr1-Kcr4). Host and symbiont phylogenies were highly congruent corroborating their co-divergence, consistent with host-symbiont interdependent metabolism and symbiont reduced genomes shaped by a long coevolutionary history. We compared the diversity ofAngomonas/symbionts from three genera of blowflies,Lucilia, Chrysomyaan...Continue Reading

References

Oct 1, 1974·The Journal of protozoology·M H MundimE W Kitajima
Feb 1, 1966·Experimental Parasitology·F G Wallace
May 1, 1981·The Journal of protozoology·E Freymuller, E P Camargo
Mar 2, 1999·Advances in Parasitology·E P Camargo
Feb 7, 2003·Nature·Mark E TorchinArmand M Kuris
Sep 1, 1961·Memórias do Instituto Oswaldo Cruz·R de MELLO
Nov 1, 1985·Parasitology Today·I Roitman, E P Camargo
Oct 24, 2006·Current Biology : CB·Nancy A Moran
Aug 22, 2007·Memórias do Instituto Oswaldo Cruz·George Poinar Jr
Aug 21, 2008·Methods in Molecular Biology·Nicholas R Markham, Michael Zuker
Nov 6, 2008·Annual Review of Genetics·Nancy A MoranAtsushi Nakabachi
Apr 29, 2009·Bioinformatics·Kévin DartyYann Ponty
Sep 11, 2009·Ecology·D W KellyD M Tompkins
Dec 23, 2009·Memórias do Instituto Oswaldo Cruz·Rosangela Aparecida RodriguesTatiana Teixeira Torres
Sep 25, 2010·PloS One·Maria Cristina Machado MottaMaria Carolina Elias
May 14, 2011·Molecular Ecology Resources·Laurent Excoffier, Heidi E L Lischer
Dec 19, 2012·Trends in Parasitology·Dmitri A MaslovJulius Lukeš
Jun 4, 2014·Molecular and Biochemical Parasitology·Julius LukešVyacheslav Yurchenko
Feb 26, 2016·Scientific Reports·Ana Carolina M JunqueiraStephan C Schuster
Mar 17, 2016·MBio·Alexei Y KostygovVyacheslav Yurchenko
Apr 23, 2016·ZooKeys·Kirstin A WilliamsMartin H Villet
May 26, 2015·Virus Evolution·Darren P MartinBrejnev Muhire

❮ Previous
Next ❯

Citations

Nov 28, 2018·The Journal of Eukaryotic Microbiology·Jan VotýpkaJulius Lukeš
Nov 18, 2019·International Journal for Parasitology·Jana KrálováAlexei Yu Kostygov

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
dissection

Software Mentioned

PAUP ∗
PONTOS
Epi Info
RAxML
AMOVA
TreeMap
VARNA
MrBayes
ClustalX
Unafold

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.