Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica ) using ISSR, SCoT and morphological markers

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Ruchi TyagiManjusha Verma

Abstract

Investigation of genetic diversity is essential for the selection of parents for crop breeding and conservation of genetic resources. To estimate the genetic variability and population structure in the midst of 45 accessions of sponge gourd brought together from different geographical areas of India, morphological traits and two molecular markers, ISSR and SCoT markers were compared. Principal components analysis of 20 morphological traits showed 72.70% variability and significant positive correlations between fruit traits. All three marker techniques clustered all accessions into two groups with few outgroups. High level of polymorphism was observed among ISSR (74.6%) and SCoT (71.5%) primers. The Bayesian model revealed the hidden grouping and showed admixture type of population. The diversity pattern is influenced by genetic marker used, as different molecular markers have different polymorphism evaluation efficiency. This study can be helpful in amplifying the genetic base and selection of specific traits for breeding. Thus, ISSR and SCoT markers are potential marker for identification in sponge gourd and provide valuable data on its genetic correlation and structure.

References

Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M Nei
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard
Sep 16, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J Souframanien, T Gopalakrishna
Jan 17, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Fatemeh PaksereshtEzzat Karami
Apr 24, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Parvin GhaffariFatemeh Keshavarzi
Aug 12, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Zahra HajibaratReza Talebi
Feb 1, 1999·Evolution; International Journal of Organic Evolution·Michael LynchJohn Colbourne

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Citations

Jan 12, 2021·Journal, Genetic Engineering & Biotechnology·Lavin KhodaeeMahmoud Khosroshahi
Oct 23, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Deepika SharmaAjay Kumar Thakur

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Methods Mentioned

BETA
PCR

Software Mentioned

Popgene
Dendrogram
NTSYS
SPSS
STRUCTURE
pc

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