Genetic diversity and silencing suppression activity of the p22 protein of Tomato chlorosis virus isolates from tomato and sweet pepper

Virus Genes
Yazmín M Landeo-RíosM Carmen Cañizares

Abstract

As for other bipartite criniviruses (genus Crinivirus, family Closteroviridae), the genome of Tomato chlorosis virus encodes an RNA silencing suppressor, the protein p22, in the 3'-proximal region of RNA1. This protein has been reported as having one of the longest lasting local suppressor activities when transiently expressed in Nicotiana benthamiana. Here, we examined the genetic diversity of the p22 gene in ToCV isolates from tomato and sweet pepper. The p22 gene sequences clearly grouped into two separated clades. However, functional analysis of both types of p22 proteins indicated no evident differences in suppressor activity. Our findings provide experimental evidence that the presence of a "strong" silencing suppressor is a conserved feature of ToCV isolates.

References

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·S W DingR H Symons
Oct 23, 1997·Nature·O Voinnet, D C Baulcombe
Mar 19, 2003·Virology·Jonathan C ReedValerian V Dolja
Apr 23, 2004·Trends in Plant Science·Dániel Silhavy, József Burgyán
Sep 17, 2004·Nature·David Baulcombe
Oct 16, 2004·The Journal of General Virology·M Carmen CañizaresGeorge P Lomonossoff
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rui LuShou-Wei Ding
Feb 11, 2005·Nature Reviews. Genetics·Olivier Voinnet
May 14, 2005·Journal of Virology·Jan F KreuzeJari P T Valkonen
Oct 21, 2005·The Plant Journal : for Cell and Molecular Biology·Elizabeth H BayneDavid C Baulcombe
Mar 15, 2006·Virus Research·Valerian V DoljaJari P T Valkonen
Dec 14, 2006·Archives of Virology·G LozanoJ Navas-Castillo
Aug 6, 2008·Annual Review of Phytopathology·Juan A Díaz-Pendón, Shou-Wei Ding

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Citations

Sep 11, 2019·Archives of Virology·Chrysoula G OrfanidouVarvara I Maliogka

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