Genetic diversity and structure of a rare endemic cactus and an assessment of its genetic relationship with a more common congener

Genetica
Niraj Rayamajhi, Jyotsna Sharma

Abstract

Endemic, obligate outcrossing plant species with narrow geographic distributions and disjunct populations are prone to loss of genetic diversity. Simultaneously, delineating clear species boundaries is important for targeted conservation efforts. The rare and endemic cactus, Sclerocactus brevihamatus subsp. tobuschii (SBT), has a parapatric relationship with Sclerocactus brevihamatus subsp. brevihamatus (SBB) but genetic distance between the two taxa is unknown. We: (1) developed taxon-specific polymorphic microsatellites, (2) assessed genetic diversity within and among nine populations of SBT, and within one population of SBB, and (3) estimated the genetic relationship between the two subspecies. Within-population genetic diversity of SBT was moderate to high (mean Ho = 0.37; mean He = 0.59). Indirect estimate of inbreeding corrected for null alleles (Fis-INEst) was low for SBT, ranging from 0.03 to 0.14 (mean Fis-INEst = 0.07). Genetic differentiation among populations of SBT was low based on Fst (0.08) and AMOVA (ФPT = 0.10). Lack of genetic and spatial correlation in SBT populations coupled with the presence of private alleles and bottleneck events in several populations suggests that reproductive isolation is occurring but...Continue Reading

References

Jul 27, 2004·Electrophoresis·Qiu-Hong WanSheng-Guo Fang
Dec 8, 2006·Molecular Biology and Evolution·Marie-Pierre Chapuis, Arnaud Estoup
Feb 1, 1996·Genome Génome / Conseil National De Recherches Canada·K Ritland
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Methods Mentioned

BETA
PCR
electrophoresis
genotyping
PCA

Software Mentioned

BOTTLENECK
Primer3
GenomeLab
MICROCHECKER
GenAlEx
RGui
RARE
HP
FreeNA
SEQBOOT

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