Genetic diversity in Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter-simple sequence repeat (ISSR) markers

Molecular Ecology
S BarthTh Lübberstedt

Abstract

In this study, we investigated genetic diversity among 37 accessions in Arabidopsis thaliana from Eurasia, North Africa and North America using morphological traits and two polymerase chain reaction (PCR)-based marker systems: cleaved amplified polymorphic sequences (CAPS) and inter-simple sequence repeats (ISSR). Cluster analysis based on genetic similarities calculated from CAPS data grouped the accessions roughly according to their geographical origin: one large group contained accessions from Western, Northern and Southern Europe as well as North Africa, a second group consisted of Eastern European and Asian continental accessions. North American accessions were interspersed into these groups. Contrary to the CAPS analysis, the dendrogram obtained from the ISSR data did not reflect the geographical origin of the accessions, and the calculated genetic distances did not match the CAPS results. This could be attributable to an uneven genomic distribution of ISSR markers as substantiated by a database search for ISSR binding sites in A. thaliana genomic DNA sequence files, or to the ISSR's different mode of evolution. We recommend CAPS markers for diversity analysis in A. thaliana because a careful selection of markers can asce...Continue Reading

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Citations

Apr 6, 2004·Plant Physiology·Rhonda C MeyerThomas Altmann
Sep 21, 2004·Annual Review of Plant Biology·Maarten KoornneefDick Vreugdenhil
Feb 16, 2013·Genes & Genetic Systems·Kok-Song LaiSeiji Takayama
Feb 9, 2012·International Journal of Molecular Sciences·Tayebeh Mohammadi FarsaniMajid Talebi
Apr 10, 2015·International Journal of Molecular Sciences·Marianna ErbanoÉlide Pereira Dos Santos
Sep 5, 2006·Forensic Science International : Synergy·Lin HeYongping Huang
Oct 29, 2013·Molecular Phylogenetics and Evolution·Ana Lúcia Anversa SegattoLoreta Brandão Freitas
Jan 6, 2015·Plant Physiology and Biochemistry : PPB·Meshack AfitlhileSamantha Workman
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Sep 19, 2019·Anais Da Academia Brasileira De Ciências·Julianna M SilvaCláudia F Ferreira
Feb 8, 2006·Genome Génome / Conseil National De Recherches Canada·A PissardP Bertin
Dec 29, 2020·Plant Physiology and Biochemistry : PPB·Meshack AfitlhileLogan Brown
Apr 29, 2004·Journal of Agricultural and Food Chemistry·Ivo Wiesner, Dana Wiesnerová

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