Genetic diversity in ex-situ conserved Lens culinaris for botanical descriptors, biochemical and molecular markers and identification of landraces from indigenous genetic resources of Pakistan

Journal of Integrative Plant Biology
Tayyaba Sultana, Abdul Ghafoor

Abstract

Lentil, one of the oldest legumes was investigated for diversity based on botanical descriptors, total seed proteins, isozymes and random amplified polymorphic DNA (RAPD) markers. About one fourth of accessions were heterogeneous for botanical descriptors and a seed protein profile. The germplasm collected from the province of Baluchistan revealed the prevalence of indigenous landraces as high diversity was observed for all of the techniques. Diversity explored through various techniques revealed validity irrespective of the sample size or geographic pattern, RAPD being the best choice for investigating both inter- and intra-accession variation in lentil. Although all of the techniques were able to resolve genetic diversity in lentil, isozymes and seed proteins gave low levels of genetic diversity, suggesting that more investigation into isozymes of specific proteins is required. RAPD is the best option for determining inter- and intra-accession variation, and will be required to extend germplasms and primers to continue the study of botanical descriptors.

References

Jun 17, 2000·Science·S Lev-YadunS Abbo
Nov 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P W Skroch, J Nienhuis
Jan 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W LinkA E Melchinger
Sep 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C E ThormannT C Osborn
Nov 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W Erskine, F J Muehlbauer
Aug 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D O SkibinskiW Erskine

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Citations

Nov 3, 2012·Plant Science : an International Journal of Experimental Plant Biology·Manuela IaliciccoGabriella S Scippa
Nov 27, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G SrinivasN Seetharama
Mar 11, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G SrinivasN Seetharama
Jan 1, 2016·3 Biotech·Dimple M PatelJigar G Mistry

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