Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

The Plant Pathology Journal
Yusuf Öztürk, Bayram Çevik

Abstract

Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed d...Continue Reading

References

Dec 1, 1978·Virology·E L Halk, R W Fulton
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jan 1, 1997·Archives of Virology·C Rampitsch, K C Eastwell
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Aug 2, 2006·Journal of Virological Methods·Hikmet Murat SipahiogluMustafa Ocak

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Citations

Aug 31, 2018·International Journal of Molecular Sciences·Edmund KoziełJózef J Bujarski

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

AlignX
Vector NTI
Clustal X2
TreeView

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