Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

BMC Veterinary Research
Marco Di DomenicoCesare Cammà

Abstract

As the epidemiology of human Q Fever generally reflects the spread of Coxiella burnetii in ruminant livestock, molecular characterization of strains is essential to prevent human outbreaks. In this study we report the genetic diversity of C. burnetii in central Italy accomplished by MST and MLVA-6 on biological samples from 20 goat, sheep and cow farms. Five MST and ten MLVA profiles emerged from the analysis establishing a part of C. burnetii strain world atlas. In particular, ST32 occurred on 12 farms (60%), prevalently in goat specimens, while ST12 (25%) was detected on 4 sheep and 1 goat samples. ST8 and a variant of this genotype were described on 2 different sheep farms, whereas ST55 was observed on a goat farm. Five complete MLVA profiles different from any other published genotypes were described in this study in addition to 15 MLVA incomplete panels. Despite this, polymorphic markers Ms23, Ms24 and Ms33 enabled the identification of samples sharing the same MST profile. Integration of such data in international databases can be of further help in the attempt of building a global phylogeny and epidemiology of Q fever in animals, with a "One Health" perspective.

References

Aug 11, 1988·The New England Journal of Medicine·J M LangleyJ C Williams
Aug 1, 1996·European Journal of Epidemiology·T Manfredi SelvaggiN Binkin
Mar 29, 2005·The Lancet Infectious Diseases·D RaoultJl Mege
Apr 9, 2005·Epidemiology and Infection·G StarniniG Rezza
Feb 1, 2006·FEMS Microbiology Letters·Sanela SvrakaWieger L Homan
Feb 3, 2007·FEMS Immunology and Medical Microbiology·Alex van Belkum
Aug 30, 2008·Infectious Disease Clinics of North America·Hervé Tissot-Dupont, Didier Raoult
May 21, 2009·FEMS Microbiology Reviews·Wenjun LiPierre-Edouard Fournier
Oct 6, 2010·Epidemiology and Infection·H I J RoestD Raoult
Apr 8, 2011·Emerging Infectious Diseases·Hendrik I J RoestFred G van Zijderveld
Nov 11, 2011·PloS One·Heidie M HornstraTalima Pearson
Dec 23, 2011·Journal of Clinical Microbiology·Jeroen J H C TilburgCorné H W Klaassen
Apr 13, 2012·Journal of Clinical Microbiology·Jeroen J H C TilburgCorné H W Klaassen
Apr 21, 2012·Emerging Infectious Diseases·Jeroen J H C TilburgCorné H W Klaassen
Jun 20, 2012·Advances in Experimental Medicine and Biology·Robert F MassungDimitrios Frangoulidis
Oct 9, 2012·International Journal of Medical Microbiology : IJMM·Ana Sofia SantosCorné H W Klaassen
Feb 20, 2013·Acta Veterinaria Scandinavica·Jørgen S Agerholm
Jun 15, 2013·Emerging Infectious Diseases·Aba MahamatDidier Raoult
Sep 13, 2014·Veterinary Microbiology·Ivana RačićZeljko Cvetnić
Oct 18, 2014·Vector Borne and Zoonotic Diseases·Marco Di DomenicoCesare Cammà
May 20, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Felicetta D'AmatoDidier Raoult
Jul 3, 2015·Vector Borne and Zoonotic Diseases·Ilaria PascucciCesare Cammà
Sep 1, 2015·Veterinary Microbiology·R Van den BromP Vellema
Sep 8, 2015·Microbes and Infection·Karim Sidi-BoumedineElodie Rousset
Aug 21, 2016·International Journal of Food Microbiology·Alessia GalieroDomenico Cerri
Nov 20, 2016·Clinical Microbiology Reviews·Carole EldinDidier Raoult

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
electrophoresis
PCR
biopsy

Software Mentioned

GeneMapper
DNASTAR Lasergene 10 Core Suite
SeqMan Pro (
Primer Express

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon saikingaku zasshi. Japanese journal of bacteriology
H Oda, K Yoshiie
Vojnosanitetski pregled. Military-medical and pharmaceutical review
M MORELJD MEL
Journal of the American Veterinary Medical Association
D Behymer, H P Riemann
© 2022 Meta ULC. All rights reserved