Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina

Memórias do Instituto Oswaldo Cruz
Regina Helena Saramago PeraltaSilvana Carnevale

Abstract

The identification and characterisation of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity of Cryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays for Cryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detecte...Continue Reading

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Citations

Feb 8, 2018·Parasitology Research·Jorge Néstor VelásquezSilvana Carnevale
Jun 13, 2019·Parasitology Research·Joaquín A LombardelliKarina I Tiranti
Sep 10, 2020·Microorganisms·Damien CostaLoïc Favennec
Jun 27, 2019·Revista Do Instituto De Medicina Tropical De São Paulo·Flavia Souza CunhaJosé Mauro Peralta
Apr 2, 2019·Veterinary Parasitology : Regional Studies and Reports·A ChikwetoR N Sharma
Feb 26, 2021·Clinical Microbiology Reviews·Xin YangYaoyu Feng
May 17, 2021·Experimental Parasitology·Jennifer K O' LearyBrigid Lucey

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Datasets Mentioned

BETA
KT381976
KT381977

Methods Mentioned

BETA
biopsy
PCR
genotyping

Software Mentioned

Bioedit Sequence Alignment Editor

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