Genetic diversity of Mycobacterium tuberculosis complex strains isolated from livestock workers and cattle in Nigeria

PloS One
Hezekiah K AdesokanSimeon Cadmus

Abstract

Molecular typing techniques are useful in understanding tuberculosis epidemiology; yet, they have been under-utilised at the human-animal interface in Nigeria. Sixty-four Mycobacterium tuberculosis complex (MTBC) isolates including 42 M. tuberculosis, 13 M. bovis and nine M. africanum obtained from livestock workers (LW, n = 47) and their cattle (n = 17) in three geographical zones of Nigeria were genotyped to identify and evaluate the genetic diversity of the circulating MTBC using spoligotyping. Distribution into clades of M. tuberculosis revealed; 45.3% Uganda I- [SIT46- cattle: 1; LW: 28], 14.1% Latin American Mediterranean- [SIT61, cattle: 1; LW: 8], and 1.6% T- [SIT53-LW: 1]. The M. bovis strains were 6.3% SB0944 [cattle: 4] and 1.6% each of SB0300, SB1026, SB1027 and SB1439 [cattle: 4]. Seventeen MTBC isolates [cattle: 7; LW: 10] yielded 14 new spoligotype patterns including three M. tuberculosis strains (three isolates), five M. bovis strains (five isolates) and six M. africanum strains (nine isolates), two of which belonged to MAF1. Only few families namely, the not previously described Uganda I-, LAM and SB0944 are predominant among the LW and cattle, with other types in lower prevalences. The strain population struct...Continue Reading

References

Jan 4, 2001·Journal of Clinical Microbiology·B M Njanpop-LafourcadeN Haddad
Jun 7, 2003·Journal of Clinical Microbiology·Sara Ngo Niobe-EyangohM Cristina Gutierrez
Mar 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Aaron E HirshPeter M Small
Jan 5, 2006·Journal of Clinical Microbiology·Simeon CadmusStephen V Gordon
May 18, 2006·Emerging Infectious Diseases·Colette Diguimbaye-DjaibéEsther Schelling
Oct 17, 2006·Clinical Microbiology Reviews·Barun MathemaBarry N Kreiswirth
Jan 26, 2007·Journal of Clinical Microbiology·S GodreuilA L Bañuls
Apr 24, 2007·The Lancet Infectious Diseases·Sebastien Gagneux, Peter M Small
Apr 9, 2008·Emerging Infectious Diseases·Melinda J WilkinsMatthew L Boulton
Oct 18, 2008·British Journal of Sports Medicine·S O Azubuike, O H Okojie
Mar 6, 2010·Tropical Animal Health and Production·Simeon I B CadmusDick van Soolingen
Jun 29, 2010·BMC Infectious Diseases·Agatha AniUlf R Dahle
Jul 14, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Edgar AbadiaChristophe Sola
Apr 5, 2011·Veterinary Microbiology·A O JenkinsJ Godfroid
May 3, 2011·PloS One·Ramona GroenheitGunilla Källenius
Aug 23, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Noel H Smith, Paul Upton
Mar 1, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe DemayNalin Rastogi
Apr 28, 2012·Veterinary Microbiology·Monika KrajewskaKrzysztof Szulowski
Sep 2, 2014·Nature Communications·Francesc CollTaane G Clark
Jan 9, 2015·PLoS Neglected Tropical Diseases·Adwoa Asante-PokuSebastien Gagneux

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
scraping

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved