Genetic Diversity of Pineapple (Ananas comosus ) Germplasm in Malaysia Using Simple Sequence Repeat (SSR) Markers

Tropical Life Sciences Research
Siti Norhayati IsmailRozlaily Zainol

Abstract

Assessments of genetic diversity have been claimed to be significantly efficient in utilising and managing resources of genetic for breeding programme. In this study, variations in genetic were observed in 65 pineapple accessions gathered from germplasm available at Malaysian Agriculture Research and Development Institute (MARDI) located in Pontian, Johor via 15 markers of simple sequence repeat (SSR). The results showed that 59 alleles appeared to range from 2.0 to 6.0 alleles with a mean of 3.9 alleles per locus, thus displaying polymorphism for all samples at a moderate level. Furthermore, the values of polymorphic information content (PIC) had been found to range between 0.104 (TsuAC035) and 0.697 (Acom_9.9), thus averaging at the value of 0.433. In addition, the expected and the observed heterozygosity of each locus seemed to vary within the ranges of 0.033 to 0.712, and from 0.033 to 0.885, along with the average values of 0.437 and 0.511, respectively. The population structure analysis via method of delta K (ΔK), along with mean of L (K) method, revealed that individuals from the germplasm could be divided into two major clusters based on genetics (K = 2), namely Group 1 and Group 2. As such, five accessions (Yankee, SRK...Continue Reading

References

Feb 12, 2005·Bioinformatics·Kejun Liu, Spencer V Muse
Jun 1, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Tina Wöhrmann, Kurt Weising
Jul 31, 2012·Plant Methods·Zhanguo Xin, Junping Chen
Jan 24, 2013·Breeding Science·Moriyuki ShodaToshiya Yamamoto
Sep 12, 2013·BioMed Research International·Suping FengYaoting Wu
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Checker
MARDI
STRUCTURE
MEGA
CONVERT
Power Marker
- Checker
Micro
POPGENE
GeneMapper

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