Genetic diversity of Pneumocystis carinii f. sp. hominis based on variations in nucleotide sequences of internal transcribed spacers of rRNA genes.

Journal of Clinical Microbiology
Laila F NimriCharles B Beard

Abstract

A variety of genes have been used to type Pneumocystis carinii. In the present study, nucleotide sequence variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were used to type Pneumocystis carinii f. sp. hominis DNA obtained from the lungs of 60 human immunodeficiency virus-infected individuals. These regions were amplified by PCR, cloned, and sequenced. Multibase polymorphisms were identified among samples. Several new genotypes are reported on the basis of the nucleotide sequence variations at previously unreported positions of both the ITS1 and the ITS2 regions. Twelve new ITS1 sequences were observed, in addition to the nine sequence types reported previously. The most common was type E, which was observed in 60.5% of the samples. The sequence variations in the ITS1 region were mainly located at positions 5, 12, 23, 24, 45, 53, and 54. Sixteen new ITS2 types were also identified, in addition to the 13 types reported previously. The most common was type g (26.6%). The sequences of the ITS2 regions in most specimens were different from the previously published sequence at bases 120 and 166 through 183. The most common variations observed were deletions at positions 177 through 183. The ...Continue Reading

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Citations

Jan 18, 2005·Transactions of the Royal Society of Tropical Medicine and Hygiene·Robert F MillerAnn E Wakefield
May 4, 2004·Molecular Phylogenetics and Evolution·Jacques GuillotRené Chermette
Nov 26, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Olga MatosFrancisco Antunes
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Apr 17, 2009·Journal of Clinical Microbiology·Sebastiaan J van HalJock Harkness
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Feb 4, 2006·Journal of Clinical Microbiology·Brice RotureauBernard Carme
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Jan 20, 2016·Fungal Biology·Patrizia DanesiSybren G de Hoog
Mar 23, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jessica BeserVictor Fernandez
Oct 19, 2010·Science China. Life Sciences·XianMin FengSiqi Lu
May 25, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rashmi GuptaVishnubhatla Sreenivas
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Jun 15, 2018·Clinical Microbiology Reviews·Liang MaJoseph A Kovacs
Jan 23, 2004·The Journal of Eukaryotic Microbiology·Robert F MillerAnn E Wakefield

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