Genetic diversity of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis revealed by restriction fragment length polymorphism of the rRNA gene region.

Journal of Clinical Microbiology
C MartinR Quentin

Abstract

The restriction fragment length polymorphism patterns of rDNAs from Pseudomonas aeruginosa strains isolated from the respiratory tracts of patients suffering from cystic fibrosis were obtained to evaluate the genetic polymorphism of this population of strains. Eighty-seven P. aeruginosa strains isolated from 87 patients from diverse areas of France and the ATCC 10145 strain were examined. Four restriction enzymes were used: BamHI, ClaI, EcoRI, and PstI. Forty-nine strains (56%) were in the three most frequent ribotypes (ribotypes R1 to R3). In addition, hierarchical clustering analysis of the data showed that 71 of the 88 strains (81%) clustered at a distance of less than one-third of the greatest distance observed in the total population. This indicates that clinical isolates implicated in the pathology of cystic fibrosis present a low degree of heterogeneity of rRNA operons, in contrast to the heterogeneity of strains of P. aeruginosa isolated from patients with various other pathologies. This relative homogeneity of rRNA genes was observed independently of the clinical status of the patient and the colony morphology.

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Citations

Aug 20, 2004·Journal of Bacteriology·Stephen E LizewskiMichael J Schurr
Apr 26, 2008·Journal of Medical Microbiology·Gordon McAlesterJohn P Morrissey
Apr 16, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Baoleri LeeOana Ciofu
May 16, 2008·International Endodontic Journal·K L BartieM A O Lewis
Nov 18, 2004·Journal of Bacteriology·Jeremy S WebbStaffan Kjelleberg
Jul 12, 2005·Proceedings. Biological Sciences·Michael A BrockhurstPaul B Rainey
Mar 6, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G H Bowden, I R Hamilton
Mar 26, 2021·Infection and Drug Resistance·Shaymaa H Abdel-Rhman, Dina E Rizk
Aug 12, 2000·Science·J E LeClerc, T A Cebula

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