Genetic diversity of symbiotic bacteria nodulating common bean (Phaseolus vulgaris) in western Kenya

PloS One
Fanuel KawakaJohn Muoma

Abstract

Biological nitrogen fixation (BNF) in legumes plays a critical role in improving soil fertility. Despite this vital role, there is limited information on the genetic diversity and BNF of bacteria nodulating common bean (Phaseolus vulgaris L.). This study evaluated the genetic diversity and symbiotic nitrogen fixation of bacteria nodulating common bean in soils of Western Kenya. The genetic diversity was determined using 16S rRNA gene partial sequences while BNF was estimated in a greenhouse experiment. The sequences of the native isolates were closely affiliated with members from the genera Pantoea, Klebsiella, Rhizobium, Enterobacter and Bacillus. These results show that apart from rhizobia, there are non-rhizobial strains in the nodules of common bean. The symbiotic efficiency (SE) of native isolates varied and exhibited comparable or superior BNF compared to the local commercial inoculants (CIAT 899 and Strain 446). Isolates (MMUST 003 [KP027691], MMUST 004 [KP027687], MMUST 005 [KP027688], KSM 001 [KP027682], KSM 002 [KP027680], KSM 003 [KP027683] and KSM 005 [KP027685]) recorded equal or significantly higher SE (p < 0.05) compared to N supplemented treatments. The results demonstrate the presence of genetic diversity of na...Continue Reading

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Citations

Apr 22, 2020·World Journal of Microbiology & Biotechnology·Abdelaal Shamseldin, Encarna Velázquez
Jan 11, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Sevil BasbugaCanan Can

Related Concepts

Diazotrophy
RNA, Bacterial
RNA, Ribosomal, 16S
Mutualism
Phaseolus acutifolius
Root Nodules, Plant
Biological Factors
Enterobacter
Fertility
Genes

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