Genetic Diversity through the Looking Glass: Effect of Enrichment Bias.

Applied and Environmental Microbiology
J DunbarL J Forney

Abstract

The effect of enrichment bias on the diversity of 2,4-dichlorophenoxyacetate (2,4-D)-degrading (2,4-D(sup+)) bacteria recovered from soil was evaluated by comparing the diversity of isolates obtained by direct plating to the diversity of isolates obtained from 85 liquid batch cultures. By the two methods, a total of 159 isolates were purified from 1 g of soil and divided into populations based on repeated extragenic palindromic sequence PCR (rep-PCR) genomic fingerprints. Approximately 42% of the direct-plating isolates hybridized with the tfdA and tfdB genes from Alcaligenes eutrophus JMP134(pJP4), 27% hybridized with the tfdA and tfdB genes from Burkholderia sp. strain RASC, and 30% hybridized with none of the probes. In contrast, the enrichment isolates not only represented fewer populations than the isolates obtained by direct plating but also exhibited, almost exclusively, a single hybridization pattern with 2,4-D catabolic gene probes. Approximately 98% of the enrichment isolates possessed pJP4-type tfdA and tfdB genes, whereas isolates containing RASC-type tfdA and tfdB genes were obtained from only 2 of the 85 enrichment cultures. The skewed occurrence of the pJP4-type genes among the isolates obtained by enrichment sug...Continue Reading

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Citations

Oct 6, 1999·FEMS Microbiology Ecology·D J SaulP L Bergquist
Jun 27, 2001·Applied and Environmental Microbiology·L KuaiM F Polz
Jun 29, 2002·Applied and Environmental Microbiology·Yasuhiro OdaLarry J Forney
Aug 7, 2003·Applied and Environmental Microbiology·Julian R Marchesi, Andrew J Weightman
Nov 5, 2005·Applied and Environmental Microbiology·Boris WawrikGerben Zylstra
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May 1, 2004·FEMS Microbiology Ecology·Geneviève L Grundmann
Nov 18, 2014·Frontiers in Microbiology·Joanita SulaimanKok-Gan Chan
Feb 2, 2002·Applied and Environmental Microbiology·Manabu YamasakiYoko Katayama
Nov 27, 2002·Applied and Environmental Microbiology·Lisa C StrongLawrence P Wackett
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Jun 6, 2003·Applied and Environmental Microbiology·Brett R BaldwinLoring Nies
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