Genetic engineering combined with deep UV resonance Raman spectroscopy for structural characterization of amyloid-like fibrils

Journal of the American Chemical Society
Vitali SikirzhytskiIgor K Lednev

Abstract

Elucidating the structure of the cross-beta core in large amyloid fibrils is a challenging problem in modern structural biology. For the first time, a set of de novo polypeptides was genetically engineered to form amyloid-like fibrils with similar morphology and yet different strand length. Differential ultraviolet Raman spectroscopy allowed for separation of the spectroscopic signatures of the highly ordered beta-sheet strands and turns of the fibril core. The relationship between Raman frequencies and Ramachandran dihedral angles of the polypeptide backbone indicates the nature of the beta-sheet and turn structural elements.

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Citations

Jun 22, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Susmita BhattacharyaAnushree Roy
Feb 18, 2012·Chemical Reviews·Sulayman A OladepoIgor K Lednev
Aug 28, 2010·The Journal of Physical Chemistry. B·Jun JiangShaul Mukamel
Jun 12, 2012·The Journal of Physical Chemistry. B·Victor ShashilovIgor K Lednev
Jun 10, 2010·Chemical Communications : Chem Comm·Dmitry KurouskiIgor K Lednev
Jan 14, 2012·Chemical Communications : Chem Comm·Dmitry KurouskiIgor K Lednev
Jun 1, 2018·Trends in Analytical Chemistry : TRAC·Ryan S JakubekIgor K Lednev
Apr 22, 2010·Journal of the American Chemical Society·Ludmila A PopovaIgor K Lednev
Mar 8, 2011·The Journal of Physical Chemistry Letters·Sulayman A OladepoSanford A Asher
Feb 4, 2014·Journal of the American Chemical Society·Dmitry KurouskiLaurence A Nafie
Oct 19, 2013·The Journal of Physical Chemistry. B·Susmita BhattacharyaAnushree Roy

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