PMID: 9663332Jul 15, 1998Paper

Genetic engineering of glucose-stimulated insulin secretion in Chinese hamster ovary cells

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
E KintsurashviliA M Sun

Abstract

To engineer an a non-islet cell capable of glucose-stimulated insulin secretion, a chinese hamster ovary cell line (CHO) was transfected with a mammalian expression vector carrying the human insulin cDNA (pCB/hINS). More proinsulin than insulin was released daily by the stably transformed cell line (CHO-INS). Examination of acid-ethanol extracts confirmed that both insulin and proinsulin were stored. Immunohistochemical analysis of the cells also showed that (pro)insulin was stored. Unlike beta cells, CHO-INS cells did not secrete insulin in response to glucose. To investigate this lack of effect, we examined whether transfection of GLUT2 cDNA, which is ordinarily not expressed in CHO-INS cells, would confer glucose-stimulated insulin secretion. Consequently, we have demonstrated that glucose regulated insulin release occurs in the CHO-INS-GLUT2 cell line and that glucose potentiates the insulin secretory response to non-glucose secretagogues.

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Citations

Nov 13, 2008·Cytotechnology·Patrick GammellMartin Clynes
Sep 1, 2000·Seminars in Cell & Developmental Biology·M MolineteP A Halban
Sep 5, 2002·Journal of Biomedical Materials Research·Paul de VosHenk J Busscher
Nov 16, 2004·Molecular and Cellular Endocrinology·Hans E Hohmeier, Christopher B Newgard

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