Genetic engineering of proteins with cell membrane permeability

Nature Biotechnology
M RojasY Z Lin

Abstract

The discovery of methods for generating proteins with inherent cell membrane-translocating activity will expand our ability to study and manipulate various intracellular processes in living systems. We report a method to engineer proteins with cell-membrane permeability. After a 12-amino acid residue membrane-translocating sequence (MTS) was fused to the C-terminus of glutathione S-transferase (GST), the resultant GST-MTS fusion proteins were efficiently imported into NIH 3T3 fibroblasts and other cells. To explore the applicability of this nondestructive import method to the study of intracellular processes, a 41-kDa GST-Grb2SH2-MTS fusion protein containing the Grb2 SH2 domain was tested for its effect on the epidermal growth factor (EGF)-stimulated signaling pathway. This fusion protein entered cells, formed a complex with phosphorylated EGF receptor (EGFR), and inhibited EGF-induced EGFR-Grb2 association and mitogen-activated protein kinase activation.

References

Sep 1, 1992·Neuron·J Schlessinger, A Ullrich
Aug 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·C SchindlerJ E Darnell
Jan 1, 1987·Annual Review of Biochemistry·G Carpenter
Aug 1, 1994·Molecular and Cellular Biology·A G BatzerJ Schlessinger
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·X Y LiuJ Hawiger
May 29, 1997·Biochemical and Biophysical Research Communications·M RojasY Z Lin
Aug 29, 1997·The Journal of Biological Chemistry·E J WilliamsP Doherty
Feb 12, 1998·Science·T Pawson, J D Scott
Apr 8, 1998·The Journal of Peptide Research : Official Journal of the American Peptide Society·C DuY Z Lin

❮ Previous
Next ❯

Citations

Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Iring HeislerHendrik Fuchs
Sep 24, 2004·Journal of Molecular Recognition : JMR·Johannes OehlkeMichael Bienert
Jun 19, 2001·Journal of Immunological Methods·Y ZhaoH Kohler
Feb 26, 2000·Trends in Pharmacological Sciences·M LindgrenU Langel
Jan 9, 2001·Trends in Biotechnology·J Gariépy, K Kawamura
Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y RojanasakulD Toledo
May 30, 2001·Advanced Drug Delivery Reviews·A MuratovskaM P Murphy
Feb 5, 2003·Advanced Drug Delivery Reviews·Ching-Hsuan Tung, Ralph Weissleder
Mar 4, 2000·Advanced Drug Delivery Reviews·M P Murphy, R A Smith
Oct 30, 2001·Critical Reviews in Oncology/hematology·M VidalC Garbay
Feb 18, 1999·Current Opinion in Chemical Biology·J Hawiger
Mar 3, 2004·Biochemistry·Stephen M Fuchs, Ronald T Raines
May 21, 1998·Nature Biotechnology·T Fernandez, H Bayley
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Yan WuFumiaki Katagiri
Sep 21, 2000·European Journal of Biochemistry·A SchellerJ Oehlke
Oct 28, 2009·The Journal of Biological Chemistry·Falk DuchardtRoland Brock
Jan 16, 1999·The EMBO Journal·S M Fujihara, S G Nadler
Jun 22, 2006·Journal of Bacteriology·Valerie J BuslerTimothy L Cover
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael PeitzFrank Edenhofer
May 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicholas KushnerYichen Lu
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lidija CovicAthan Kuliopulos
Nov 26, 2013·Advanced Drug Delivery Reviews·Swati Biswas, Vladimir P Torchilin
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·L ZhangJ P Tam
Oct 16, 2004·Molecular and Cellular Neurosciences·Gunnar P H Dietz, Mathias Bähr
Mar 11, 2005·Expert Opinion on Biological Therapy·Sybille MullerHeinz Kohler
Nov 26, 2010·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Daniel A GroveMauricio Rojas
Dec 15, 2015·Biochimica Et Biophysica Acta·Remigiusz WorchMarzena Jankowska-Anyszka
Dec 7, 2007·Advanced Drug Delivery Reviews·Vladimir P Torchilin
Jan 29, 2008·British Journal of Pharmacology·T SugitaY Tsutsumi
Aug 13, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Siegmund Reissmann
Sep 25, 2007·Biochimica Et Biophysica Acta·Kamel MabroukMichel De Waard
Dec 3, 2004·Drug Discovery Today·Jamal Temsamani, Pierre Vidal
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Rachel Tréhin, Hans P Merkle
Oct 4, 2005·Biochemical and Biophysical Research Communications·Christopher BachranMark Sutherland
Apr 7, 2016·Cellular and Molecular Life Sciences : CMLS·Stefan KrautwaldUlrich Kunzendorf
Jun 15, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Annarita FalangaStefania Galdiero
Mar 12, 2002·Virology·Nico MichelMartin Müller
Jul 8, 1998·Chemistry & Biology·C Denison, T Kodadek
Mar 15, 2008·Biochimica Et Biophysica Acta·Tatiana B StoilovaVadim T Ivanov
Aug 10, 2000·Biochimica Et Biophysica Acta·U SoometsU Langel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.