Genetic environment of metallo-β-lactamase genes in Pseudomonas aeruginosa isolates from the UK

The Journal of Antimicrobial Chemotherapy
Laura L WrightNeil Woodford

Abstract

We sought to characterize the genetic environment of blaVIM and blaIMP genes in Pseudomonas aeruginosa isolates from the UK; these included members of six previously described prevalent complexes, A-F, which correspond to international 'high-risk clones', along with diverse strains. Metallo-β-lactamase (MBL)-encoding class 1 integrons were amplified by PCR from 218 P. aeruginosa isolates producing VIM-type (n = 196) or IMP-type (n = 22) enzymes, referred from UK hospital laboratories between 2003 and 2012. The variable regions of selected integrons were sequenced using a primer walking method. One-hundred-and-nineteen isolates had an MBL-encoding integron with the 3' conserved sequence (3'CS), 65 had Tn5090-like 3' regions and 17 had the sul1 gene, but lacked the qacEΔ1 gene; the 3' region could not be amplified using any primer combinations for the remaining 17 isolates. Six integron profiles were each seen in more than five isolates. Predominant integron types were seen amongst isolates belonging to STs 111, 233, 654/964 and 773 (complexes A, C, D and F, respectively), whereas diverse integron profiles were seen in isolates belonging to ST235 (complex B) and ST357 (complex E). In UK P. aeruginosa isolates, MBL genes occur in ...Continue Reading

References

Jan 1, 1995·Antimicrobial Agents and Chemotherapy·C LévesqueP H Roy
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
May 25, 2004·Antimicrobial Agents and Chemotherapy·Tse Hsien KohLi-Hwei Sng
Jul 8, 2005·Journal of Clinical Microbiology·Johann D D PitoutDeirdre L Church
Sep 30, 2008·The Journal of Antimicrobial Chemotherapy·Neil WoodfordDavid M Livermore
Feb 19, 2009·Antimicrobial Agents and Chemotherapy·Wojciech DuljaszCrista Jebelean
Aug 14, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M CastanheiraR N Jones
Mar 30, 2012·The Journal of Antimicrobial Chemotherapy·H W StokesSteven Djordjevic
Dec 22, 2012·The Lancet Infectious Diseases· The Lancet Infectious Diseases
Jul 12, 2013·The Journal of Antimicrobial Chemotherapy·Moon Jung KimKyungwon Lee
Jul 28, 2013·The Journal of Antimicrobial Chemotherapy·Katy JeannotPatrick Plésiat
Sep 4, 2014·The Journal of Antimicrobial Chemotherapy·Laura L WrightNeil Woodford
Nov 5, 2014·Antimicrobial Agents and Chemotherapy·Veronica N KosHumphrey Gardner
Jan 22, 2015·Antimicrobial Agents and Chemotherapy·Adriana CorreaMaria V Villegas

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Citations

Apr 26, 2017·Antimicrobial Agents and Chemotherapy·Rongmin ZhangYongning Wu

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