Genetic evidence for a role for protein kinase A in the maintenance of sleep and thalamocortical oscillations.

Sleep
Kevin HellmanT Abel

Abstract

Genetic manipulation of cAMP-dependent protein kinase A (PKA) in Drosophila has implicated an important role for PKA in sleeplwake state regulation. Here, we characterize the role of this signaling pathway in the regulation of sleep using electroencephalographic (EEG) and electromyographic (EMG) recordings in R(AB) transgenic mice that express a dominant negative form of the regulatory subunit of PKA in neurons within cortex and hippocampus. Previous studies have revealed that these mutant mice have reduced PKA activity that results in the impairment of hippocampus-dependent long-term memory and long-lasting forms of hippocampal synaptic plasticity. PKA assays, in situ hybridization, immunoblots, and sleep studies were performed in R(AB) transgenic mice and wild-type control mice. We have found that R(AB) transgenic mice have reduced PKA activity within cortex and reduced Ser845 phosphorylation of the glutamate receptor subunit GluR1. R(AB) transgenic mice exhibit non-rapid eye movement (NREM) sleep fragmentation and increased amounts of rapid eye movement (REM) sleep relative to wild-type mice. Further, R(AB) transgenic mice have more delta power but less sigma power during NREM sleep relative to wild-type mice. After sleep de...Continue Reading

References

Nov 1, 1991·Journal of Clinical and Experimental Neuropsychology·M A BédardJ Malo
May 1, 1981·Electroencephalography and Clinical Neurophysiology·A A BorbélyD Lehmann
Jan 1, 1982·Sleep·M HirshkowitzI Karacan
Feb 1, 1997·Physiology & Behavior·B D YoungbloodR B Harris
Aug 13, 1998·Pharmacology, Biochemistry, and Behavior·Z LelkesG Benedek
Sep 29, 1998·Electroencephalography and Clinical Neurophysiology·A BessetM Billiard
Oct 9, 1998·Journal of Neuroscience Methods·J Muthuswamy, N V Thakor
Jul 15, 1999·Nature Neuroscience·A Lüthi, D A McCormick
Oct 12, 2000·Journal of Neurophysiology·R HuberI Tobler
Mar 16, 2001·Trends in Neurosciences·L GravesT Abel
Apr 20, 2001·JAMA : the Journal of the American Medical Association·J D EdingerR E Quillian
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·P MeerloF W Turek
Nov 3, 2001·Science·R StickgoldM Fosse
Apr 6, 2002·Journal of Clinical and Experimental Neuropsychology·Cynthia F SalorioMatthew L Uhles
May 25, 2002·Chronobiology International·Niki LindblomMaija-Liisa Laakso
Aug 7, 2002·Journal of Neurophysiology·I G CampbellJ M Horowitz
Sep 5, 2002·Nature Reviews. Neuroscience·J Allan Hobson, Edward F Pace-Schott
Sep 11, 2002·Psychological Science·Staffan HyggeMonika Bullinger
Sep 28, 2002·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Kate CrowleyIan M Colrain
Dec 6, 2002·Journal of Neurophysiology·Christopher L DouglasRalph Lydic
Feb 21, 2003·Journal of Child Neurology·Yael LeitnerShaul Harel
May 10, 2003·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·B OndzeA Besset
Nov 1, 2003·Sleep Medicine·Sari Leena HimanenJoel Hasan
Sep 29, 2004·Neuron·Robert P Vertes
Jun 21, 2005·Neuroscience·M P WalkerG Schlaug
Mar 7, 2006·Annals of Clinical Psychiatry : Official Journal of the American Academy of Clinical Psychiatrists·Russell P Rosenberg
Mar 21, 2006·Seizure : the Journal of the British Epilepsy Association·Ramin KhatamiClaudio L Bassetti

❮ Previous
Next ❯

Citations

Jul 20, 2012·Physiological Reviews·Ritchie E BrownRobert W McCarley
Jan 16, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Alisson Menezes Araujo LimaPedro Felipe Carvalhedo de Bruin
Jun 29, 2011·Pflügers Archiv : European journal of physiology·Urs Albrecht
Jul 2, 2021·Neurobiology of Sleep and Circadian Rhythms·Rama DibValérie Mongrain
Sep 21, 2013·Learning & Memory·Toni-Moi Prince, Ted Abel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.