Genetic evidence for a role of phospholipase C at the budding yeast kinetochore

Molecular Genetics and Genomics : MGG
Nicholas DeLilloAles Vancura

Abstract

Chromosome segregation during mitosis requires kinetochores, specialized organelles that mediate chromosome attachment to spindle microtubules. We have shown previously that in budding yeast, Plc1p (phosphoinositide-specific phospholipase C) localizes to centromeric loci, associates with the kinetochore proteins Ndc10p and Cep3p, and affects the function of kinetochores. Deletion of PLC1 results in nocodazole sensitivity, mitotic delay, and a higher frequency of chromosome loss. We report here that despite the nocodazole sensitivity of plc1Delta cells, Plc1p is not required for the spindle checkpoint. However, plc1Delta cells require a functional BUB1/BUB3-dependent spindle checkpoint for viability. PLC1 displays strong genetic interactions with genes encoding components of the inner kinetochore, including NDC10, SKP1, MIF2, CEP1, CEP3, and CTF13. Furthermore, plc1Delta cells display alterations in chromatin structure in the core centromere. Chromatin immunoprecipitation experiments indicate that Plc1p localizes to centromeric loci independently of microtubules, and accumulates at the centromeres during G(2)/M stage of cell cycle. These results are consistent with the view that Plc1p affects kinetochore function, possibly by mo...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·F Spencer, P Hieter
Aug 9, 1991·Cell·R Li, A W Murray
Jan 1, 1991·Methods in Enzymology·F Sherman
Nov 1, 1990·Molecular and Cellular Biology·M J SaundersK Bloom
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M SaundersK Bloom
Jun 1, 1988·Molecular and Cellular Biology·J H HegemannP Hieter
Jun 1, 1995·The Journal of Cell Biology·M WineyJ R McIntosh
Nov 1, 1994·The Journal of Cell Biology·P K SorgerA A Hyman
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·K Middleton, J Carbon
Jul 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J H Hegemann, U N Fleig
Aug 13, 1993·Cell·N DivechaR F Irvine
May 1, 1993·The Journal of Cell Biology·P Y Goh, J V Kilmartin
Nov 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D J LawL M Silver
Mar 1, 1996·Molecular and Cellular Biology·M M SmithP C Megee
Aug 1, 1996·Molecular Biology of the Cell·F Pangilinan, F Spencer
Dec 17, 1997·Molecular Biology of the Cell·K SchwartzD Botstein
Mar 11, 1999·Current Opinion in Genetics & Development·A Amon
Jul 31, 1999·The Journal of Cell Biology·X ZengW S Saunders

❮ Previous
Next ❯

Citations

Apr 13, 2007·Molecular Biology of the Cell·Nilanjan GuhaAles Vancura
Dec 11, 2003·Yeast
May 23, 2009·FEMS Microbiology Letters·Katarzyna GuzinskaAles Vancura
Aug 6, 2013·The Journal of Biological Chemistry·Luciano GaldieriAles Vancura
Jun 29, 2014·The Journal of Microbiology·Chun-Seob AhnJae Won Kim
Apr 6, 2005·The Journal of Biological Chemistry·Jonathan D StallingsMario J Rebecchi
Oct 22, 2005·Journal of Cellular Biochemistry·Hitoshi Yagisawa
Dec 15, 2005·The Journal of Biological Chemistry·Carlos RomeroAles Vancura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.