Genetic evidence for latent Septata intestinalis infection in human immunodeficiency virus-infected patients with intestinal microsporidiosis

The Journal of Infectious Diseases
C FranzenM Schrappe

Abstract

Sequence data of the small subunit rRNA (SSU-rRNA) gene were used to identify Septata intestinalis in biopsies of human immunodeficiency virus-infected patients by polymerase chain reaction (PCR), southern blot hybridization, cloning, and comparative genetic sequencing. DNA products of correct size could be amplified from all examined tissues with S. intestinalis infection but also from 2 biopsies with Enterocytozoon bieneusi and from 1 biopsy with Encephalitozoon cuniculi infection. Southern blot hybridization with an S. intestinalis-specific probe and partial sequencing of the DNA fragments showed high homology with published S. intestinalis sequences and confirmed that the amplified PCR products really derived from the SSU-rRNA gene of S. intestinalis. PCR testing can detect very light infections with S. intestinalis. Thus, S. intestinalis seems to occur more frequently in the form of latent infections and the true prevalence of the parasites may be much higher than previously reported.

Citations

Jan 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S Katzwinkel-WladarschH Rinder
May 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N P KockJ Schottelius
Nov 21, 1996·The New England Journal of Medicine·C FranzenG Fätkenheuer
Jul 16, 2005·Clinical Microbiology Reviews·Alexander MathisPeter Deplazes
Oct 13, 2006·Current Opinion in Infectious Diseases·A Curry
Jul 10, 2019·Acta Parasitologica·Jorge Néstor VelásquezSilvana Carnevale
Mar 6, 1999·Clinical and Diagnostic Laboratory Immunology·A MüllerC Franzen
Dec 1, 1996·Journal of Clinical Microbiology·J N VelásquezM I Rodríguez

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