PMID: 375026Mar 20, 1979Paper

Genetic evidence for the nature, and excision repair, of DNA lesions resulting from incorporation of 5-bromouracil

Molecular & General Genetics : MGG
M KrychD Shugar

Abstract

Escherichia coli mutants defective in DNA uracil N-glycosidase (ung-) or endonuclease VI active against apurinic/apyrimidinic sites in DNA (xthA-) exhibit enhanced sensitivity towards 5-bromodeoxyuridine relative to the wild type strain, pointing to involvement of these enzymes in repair of bromouracil-induced lesions in DNA. Mutants defective in DNA polymerase I, either in polymerizing activity (polAl-) or (5' leads to 3')-exonuclease activity (polA107-) exhibit unusually high sensitivity (including marked lethality) in the presence of 5-bromodeoxyuridine. The results indicate that DNA polymerase I, and its associated (5'--3')-exonuclease activity, are involved in repair of bromouracil-induced lesions and are not readily replaced, if at all, by DNA polymerases II and III. Thermosensitive mutant in DNA ligase gene (lig ts7) shows high sensitivity towards 5-bromodeoxyuridine at 42 degrees C indicating the role of the enzyme in repair of bromouracil-induced lesions in DNA. Involvement of DNA uracil N-glycosidase, and endonuclease active against apurinic/apyrimidinic sites in recognition and repair of 5-bromouracil-induced damage permits of some inferences regarding the nature of this damage (lesions), in particular dehalogenation...Continue Reading

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·K Bebenek, C Janion
Apr 1, 1980·Biochemical Pharmacology·W G Verly
May 1, 1985·Mutation Research·I PietrzykowskaD Shugar
Jan 25, 1980·Nucleic Acids Research·J A SiedleckiB Zmudzka
Nov 15, 2012·Genetics·Sarah A SabatinosSusan L Forsburg
Dec 13, 2006·Mutation Research·David M Wilson, Larry H Thompson
Jun 1, 1980·Chemico-biological Interactions·G P MargisonL Tomatis
Jun 29, 2011·Angewandte Chemie·Philippe MarlièreRupert Mutzel
Mar 1, 1981·Critical Reviews in Toxicology·P F Schendel
Jan 1, 1988·Environmental and Molecular Mutagenesis·S S Wallace
Jul 25, 1982·Journal of Molecular Biology·T R Skopek, F Hutchinson

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