Genetic evidence for transcriptional activation by the yeast IME1 gene product.

Genetics
H E SmithA P Mitchell

Abstract

IME1 is required in yeast for meiosis and for expression of IME2 and other early meiotic genes. IME1 is a 360-amino acid polypeptide with central and C-terminal tyrosine-rich regions. We report here that a fusion protein composed of the lexA DNA-binding domain and IME1 activates transcription in vivo of a reporter gene containing upstream lexA binding sites. Activation by the fusion protein shares several features with natural IME1 activity: both are dependent on the RIM11 gene product; both are impaired by the same ime1 missense mutations; both are restored by intragenic suppressors. The central tyrosine-rich region is sufficient to activate transcription when fused to lexA. Deletion of this putative activation domain results in a defective IME1 derivative. Function of the deletion derivative is restored by fusion to the acidic Herpesvirus VP16 activation domain. The C-terminal tyrosine-rich region is dispensable for transcriptional activation; rather it renders activation dependent upon starvation and RIM11. Immunofluorescence studies indicate that an IME1-lacZ fusion protein is concentrated in the nucleus. These observations are consistent with a model in which IME1 normally stimulates IME2 expression by providing a transcri...Continue Reading

Citations

Mar 1, 1994·Microbiological Reviews·A P Mitchell
Jul 3, 2020·Genes·Anne M MacKenzie, Soni Lacefield
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roy M WilliamsRochelle E Esposito
Nov 25, 2003·The Journal of Biological Chemistry·Dawn M CliffordGeorge S Brush
Feb 5, 2008·Applied and Environmental Microbiology·Manuel Ramírez, Jesús Ambrona
Nov 7, 2002·Current Genetics·Kedar Purnapatre, Saul M Honigberg
Jan 12, 2005·Eukaryotic Cell·Miguel Arévalo-Rodríguez, Joseph Heitman
Oct 1, 1995·Molecular and Cellular Biology·R A Sia, A P Mitchell
May 1, 1997·Molecular and Cellular Biology·S Vidan, A P Mitchell
Jun 1, 1995·Molecular and Cellular Biology·K S BowdishA P Mitchell
Dec 1, 1994·Molecular and Cellular Biology·K S BowdishA P Mitchell
Jul 22, 1998·Molecular and Cellular Biology·S M Honigberg, R H Lee
Jan 11, 2000·Molecular and Cellular Biology·D DavisA P Mitchell
Sep 30, 2010·Molecular and Cellular Biology·Natalie StrudwickMartin Schröder
Apr 1, 1994·Genes & Development·R StrichR E Esposito

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