Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis.

Developmental Cell
Yan LiBin Zhou

Abstract

Genetic lineage tracing unravels cell fate and plasticity in development, tissue homeostasis, and diseases. However, it remains technically challenging to trace temporary or transient cell fate, such as epithelial-to-mesenchymal transition (EMT) in tumor metastasis. Here, we generated a genetic fate-mapping system for temporally seamless tracing of transient cell fate. Highlighting its immediate application, we used it to study EMT gene activity from the local primary tumor to a distant metastatic site in vivo. In a spontaneous breast-to-lung metastasis model, we found that primary tumor cells activated vimentin and N-cadherin in situ, but only N-cadherin was activated and functionally required during metastasis. Tumor cells that have ever expressed N-cadherin constituted the majority of metastases in lungs, and functional deletion of N-cad significantly reduced metastasis. The seamless genetic recording system described here provides an alternative way for understanding transient cell fate and plasticity in biological processes.

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Citations

Feb 7, 2021·Nature Reviews. Cancer·Arthur W Lambert, Robert A Weinberg
Feb 14, 2021·Cancers·Eldad Zacksenhaus, Sean E Egan
Mar 8, 2021·The Journal of Biological Chemistry·Xueying Tian, Bin Zhou
May 22, 2021·Current Opinion in Cell Biology·Jacco van Rheenen, Colinda L G J Scheele
Jun 3, 2021·Journal of Clinical Medicine·Laura BornesJacco van Rheenen
Nov 1, 2020·Life Sciences·Cheryl Qian Ying Yong, Bor Luen Tang
Sep 16, 2020·Developmental Cell·Pauline Vieugué, Cédric Blanpain

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