Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish

Journal of Applied Microbiology
C R AriasP H Klesius

Abstract

To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragment length polymorphism (AFLP) fingerprinting. Thirty Fl. columnare cultures isolated from different fish species and geographical origins as well as reference strains were included in the study. Fifteen isolates belonged to genomovar I while eleven were ascribed to genomovar II. Analysis of the ISR sequence confirmed the genetic differences between both genomovars but revealed a higher diversity among genomovar I isolates. The maximum resolution was provided by AFLP fingerprinting, as up to 22 AFLP profiles could be defined within the species. We confirmed the division of Fl. columnare isolates from cultured fish into different genogroups. We showed that both genomovars I and II are present in channel catfish from the US. We described a unique genetic group represented by four Fl. columnare isolates from tilapia in Brazil which appears to be related to both genomovars. We were able to further subdivide the species by analysing the ISR. Fi...Continue Reading

Citations

Oct 1, 2010·Indian Journal of Microbiology·Lopamudra SahooS C Mukherjee
Feb 12, 2010·The ISME Journal·Beltran Rodriguez-BritoForest Rohwer
Jul 16, 2013·Applied and Environmental Microbiology·Wenlong CaiCovadonga R Arias
Aug 28, 2012·Applied and Environmental Microbiology·Zhen TaoCovadonga R Arias
Apr 27, 2012·Journal of Bacteriology·Hasan C TekedarMark L Lawrence
Jul 16, 2008·BMC Microbiology·Andrew M StaroscikDavid R Nelson
Jul 6, 2014·Diseases of Aquatic Organisms·Haitham H Mohammed, Covadonga R Arias
Aug 16, 2011·Parasitology International·Stephen A BullardCovadonga R Arias
Jan 27, 2009·Journal of Microbiological Methods·Benjamin R LaFrentz, Phillip H Klesius
Aug 21, 2007·Systematic and Applied Microbiology·Oscar Olivares-FusterCovadonga R Arias
Apr 9, 2005·Journal of Fish Diseases·H C P FigueiredoM T D Peixoto
Mar 21, 2008·Journal of Fish Diseases·O Olivares-FusterC R Arias
Aug 13, 2015·Journal of Fish Diseases·H H Mohammed, C R Arias
May 28, 2016·Genome Announcements·Salih KumruMark L Lawrence
Nov 10, 2010·Fish & Shellfish Immunology·Craig A ShoemakerJoyce J Evans
Sep 3, 2016·Genome Announcements·Yulei ZhangLi Lin
Jul 29, 2008·Brazilian Journal of Biology = Revista Brasleira De Biologia·F PilarskiP S Ceccarelli

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